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铝佐剂对HBoV1 VP2 VLPs诱导小鼠免疫应答的影响 被引量：2

Effect of alum on immune response in mice induced by HBoV1 VP2 VLPs
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摘要 目的:探讨铝佐剂对HBoV1 VP2 VLPs诱导小鼠免疫应答的影响。方法:BABL/c小鼠随机分为VLPs实验组、明矾佐剂实验组、PBS对照组和明矾佐剂对照组。实验组小鼠分别采用HBoV1 VP2 VLPs和HBoV1 VP2 VLPs加明矾佐剂肌肉注射免疫,对照组小鼠同期注射等量明矾佐剂或PBS缓冲液;实验8周后ELISA检测两实验组特异性Ig G抗体效价和活性,ELIspot检测两实验组特异性细胞免疫反应强度,从细胞免疫和体液免疫强度探讨铝佐剂对HBoV1 VP2 VLPs诱导小鼠免疫应答的影响。结果:明矾佐剂降低HBoV1 VP2 VLPs诱导细胞免疫反应强度(P<0.001),增强HBoV1 VP2 VLPs诱导的血清Ig G效价(P<0.01)和Ig G活性(P<0.05)。结论:明矾佐剂使HBoV1 VP2 VLPs诱导的体液免疫反应增强而细胞免疫反应减弱。HBoV1 VP2 VLPs作为预防性疫苗使用时应添加明矾佐剂,作为治疗性疫苗使用时应不加明矾佐剂。 Objective：To study the effect of alum on immune response in mice induced by HBoV1 VP2 VLPs. Methods： BABL/c mice were randomly divided into VLPs experimental group, alum adjuvant experimental group, PBS control group and alum adjuvant control group,the experimental group mice were intramuscular immunization with HBoV1 VP2 VLPs and HBoV1 VP2 VLPs added alnm, control group mice were immunization with alum or PBS buffer, then to study the effect of alum on immune response in mice induced by HBoV1 VP2 VLPs by cellular and humoral immune strength. Results： Alum adjuvant decreased cellular immune response induced by HBoV1 VP2 VLPs（P〈0. 001 ）,enhance the HBoV1 VP2 VLPs immuned serum IgG titer（P〈0. 05）and IgG activity（P〈 0. 01 ）. Conclusion： Alum adjuvant can enhance humoral immune response induced by HBoV1 VP2 VLPs, but weaken cellular immune response induced by HBoV1 VP2 VLPs, when HBoV1 VP2 VLPs used as a prophylactic vaccine it should add alum adjuvant, when used as a therapeutic vaccine, it should not add alum adjuvant.
作者 邓中华 段招军 谢志萍 谢乐云 张兵 曹友德
机构地区 湖南省人民医院检验科 中国疾病预防控制中心病毒病预防控制所
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2016年第1期56-58,64,共4页 Chinese Journal of Immunology
基金 湖南省科技厅科研条件创新专项计划(No.2012TT2004) 湖南省人民医院仁术基金
关键词 铝佐剂 人博卡病毒1(HBoV1) 病毒蛋白2(VP2) 病毒样颗粒(VLPs) Alum Human bocavirus-1 （ HBoV1 ） Viral protein 2 （ VP2 ） Virus-like particles （VLPs）
分类号 R392.7 [医药卫生—免疫学]
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参考文献16

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二级参考文献24

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中国免疫学杂志
2016年 第1期

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