服用他克莫司的肾移植患者ABCB1基因多态性与术后感染的相关性

Correlation between ABCB1 polymorphism and postoperative infection in renal transplant patients using tacrolimus

目的:观察服用他克莫司(Tacrolimus)的肾移植受者ABCB1基因多态性对于术后感染发生的影响。方法:选取行肾移植手术并服用他克莫司的患者90例,根据术后是否存在感染分为感染组和对照组,检测所有患者的ABCB1基因型3435C>T,1236C>T和2677G>A/T。采用χ2检验,方差分析和独立样本T检验的方法对患者的基本临床资料进行检验。采用卡方检验比较感染组和未感染组的基因多态性频率分布差异。结果:患者C1236T,C3435T,G2677A/T等位基因频率,均符合Hardy-Weinberg平衡。感染组和非感染组患者的年龄,性别,体质量,临床诊断,吗替麦考酚酯(Mycophenolate Mofetil)的用量均没有显著性差异。2组患者的平均血药谷浓度在一年内均无统计学差异。C1236T单核苷酸多态性对肾移植术后感染发生的影响具有统计学意义(P=0.034)。G2677A/T突变的发生对肾移植术后感染发生的影响具有统计学意义(P=0.010)。结论:ABCB1基因多态性中,仅C1236T的SNP多态性与G2677A/T的突变对肾移植术后感染发生的影响具有统计学意义。

OBJECTIVE To investigate effects of ABCB1 polymorphism on tacrolimus related infection. METItODS Ninety renal transplant patients who used tacrolimus were divided into infection group and control group. ABCB1 of C1236T, C3435T and G2677A/T polymorphism were genotyped. （2 test, one-way ANOVA test and independent sample T test were used to ana- lyze basic clinical data of patients. （2 test was used to compare ABCB1 polymorphism discrepancy between infection group and control group. RESULTS Allele frequencies of C1236T, C3435T, G2677A/T were all fitted Hardy-Weinberg equilibrium. No difference was found in age, weight, sex, clinical diagnosis or dosage of mycophenolate mofetil between infection group and control group. There was no difference in average blood trough concentration between two groups in one year. C1236T mono- nucleotide polymorphism had significant effects on infection after renal transplantation （P = 0. 034）. Mutation of G2677A/T had statistical significance on infection after operation （P = 0. 010）. CONCLUSION Polymorphism of C1236T and mutation of G2677A/T both show significant effects on tacrolimus-related infection after renal transplantation.