干扰素诱导跨膜蛋白3在肝癌中的表达及功能研究

Interferon-induced transmembrane protein3 expression and its function in hepatocellular carcinoma

目的:探讨干扰素诱导跨膜蛋白3(IFITM3)在原发性肝癌中的表达及意义。方法:收集55例配对的肝癌与癌旁组织标本,分别用免疫组化和Western blot检测组织标本中IFITM3蛋白的表达,并分析IFITM3表达与肝癌患者临床病理因素的关系;用IFITM3干扰片段转染在肝癌Hep G2细胞后,观察肝癌细胞侵袭迁移能力的变化。结果:免疫组化结果显示,IFITM3蛋白在肝癌组织中的阳性表达率明显高于癌旁组织(88.2%vs.23.5%,P<0.05),低/中分化的肝癌组织中阳性表达率高于高分化的肝癌组织(80.0%vs.8.3%,P<0.05);Western blot结果显示,肝癌组织中IFITM3蛋白表达量明显高于癌旁组织(1.2 399 vs.0.9 565,P<0.05);IFITM3表达量与门静脉癌栓形成、肿瘤大小、TNM分期有关(均P<0.05)。Hep G2细胞转染IFITM3干扰片段后,侵袭、迁移能力均明显减弱。结论:IFITM3在肝癌中表达升高,且其升高程度与肝癌细胞的侵袭、迁移能力密切相关,提示IFITM3在肝癌的恶性进展中起了重要作用。

Objective： To investigate the expression of interferon-induced transmembrane protein 3（IFITM3） in hepatocellular carcinoma（HCC） and its signii cance. Methods： Fit y-i ve specimens of paired HCC and adjacent tissues were collected, in which the IFITM3 protein expressions were determined by immunohistochemical staining and Western blot analysis, respectively, and the relations of IFITM3 expression with the clinicopathologic factors of HCC patients were also analyzed. After transfection with interfering sequences against IFITM3, the changes in invasion and migration ability in HCC HepG 2 cells were observed. Results： Results of immunohistochemical staining showed that the IFITM3 positive expression rate in HCC tissue was significantly higher than that in its adjacent tissue（88.2% vs. 23.5%, P〈0.05）, and in low/moderatedif erentiated HCC tissue was signii cantly higher than that in high dif erentiated HCC tissue（80.0% vs. 8.3%, P〈0.05）; results of Western blot analysis demonstrated that IFITM3 protein expression level in HCC tissue was significantly higher than that in its adjacent tissue（1.2 399 vs. 0.9 565, P〈0.05）; IFITM3 expression level was signii cantly associated with portal vein tumor thrombosis, tumor size and TNM stage（all P〈0.05）. Both invasion and migration abilities in Hep G2 cells were remarkably reduced after transfection with interfering sequences against IFITM3.Conclusion： IFITM3 expression is increased in HCC, and its increasing degree is closely associated with invasion and migration ability of HCC cells, which suggest that IFITM3 plays an important role in the process of malignant progression in HCC.