血必净对脓毒症急性肺损伤大鼠氧化应激及炎性因子表达的影响

The Effect of Xuebijing on the Expression of Oxidative Stress and Inflammatory Factors in Sepsis-evoked Lung Injury Rats

目的探讨血必净对脓毒症急性肺损伤大鼠氧化应激及炎性因子表达的影响。方法大鼠随机均分为三组:假手术组(C组)、脓毒症组(CLP组)和血必净组(XBJ组)。XBJ组于CLP前3 d腹腔注射血必净。CLP后24 h每组麻醉下留取大鼠肺组织。采用对应试剂盒测定大鼠肺组织ROS及SOD活性,ELISA法测定大鼠肺组织HMGB1水平。观察大鼠术后7 d的生存率。结果与C组比较,CLP组大鼠肺组织ROS活性增高,SOD活性降低,HMGB1水平升高,术后7 d的生存率明显降低(P<0.05);与CLP组比较,XBJ组大鼠肺组织ROS活性降低,SOD活性增高,HMGB1水平降低,术后7 d的生存率明显升高(P<0.05)。结论血必净在改善脓毒症大鼠生存率的同时改善脓毒症急性肺损伤炎性反应,其机制可能与降低ROS活性、提高抗氧化酶SOD活性,同时抑制炎症因子HMGB1水平有关。

Objective To investigate the effect of xuebijing on the expression of oxidative stress and inflammatory factors in sepsis-evoked lung injury rats. Methods Forty-eight Wistar rats were randomly divided into 3 groups： control group （group C）, CLP group （group CLP）, xuebijing group （group XBJ）. Septic model was performed by cecum ligation and puncture （CLP）. In group XBJ, xuebijing 10 ml/（kg x bw） was injected intraperitoneally 72 h before CLP. Rats were been given a general anesthesia 24 h after CLP, lung tissue were harvested. ROS and SOD activity of rat lung tissues were measured, ELISA was performed for the level of HMGB 1. The mortality rate was calculated 7 days after operation. Results Compared with group C, ROS activity and levels of HMGB 1 was increased, however, SOD activity was decreased in Group CLP, also, postoperative 7d survival rate decreased significantly （P 〈 0.05）; Compared with CLP Group, ROS activity and levels of HMGB1 was decreased, but SOD activity was increased in Group CLP, also, postoperative 7d survival rate increased significantly （P 〈 0.05）. Conclusion Xuebijing could improve the survival rate of septic rats, also attenuate lung inflammatory response to injury.