毒黄素对非小细胞肺癌A549细胞株作用的实验研究

Experimental study of toxoflavin on human non-small cell lung cancer cells A549

目的研究毒黄素不同浓度和不同作用时间对非小细胞肺癌A549细胞增殖、凋亡和迁移的影响。方法分别取0.5、0.375、0.25和0.125μmol/L毒黄素作用于A549细胞(实验组),以不加毒黄素为对照组,分别培养24 h和48 h。采用CCK-8试剂盒、Annexin V-FITC/PI凋亡试剂盒检测A549细胞增殖抑制率和凋亡率。划痕实验对比0.125μmol/L毒黄素组和对照组(0μmol/L毒黄素)在12、24和48 h细胞迁移率。结果 0.5、0.375、0.25和0.125μmol/L毒黄素组24 h细胞增殖抑制率分别为(93.51±3.69)%、(40.38±3.08)%、(23.54±2.58)%和(13.07±2.37)%,48 h为(90.53±3.58)%、(53.72±3.02)%、(34.44±3.10)%和(24.78±2.43)%。0.5、0.375、0.25和0.125μmol/L毒黄素组24 h细胞凋亡率分别为(78.68±2.22)%、(43.66±2.53)%、(20.81±2.59)%和(6.25±0.96)%,高于对照组的(1.57±0.52)%;0.5、0.375、0.25和0.125μmol/L毒黄素组48 h细胞凋亡率分别为(88.66±3.16)%、(59.86±2.81)%、(27.89±3.48)%和(9.91±1.33)%,高于对照组的(1.59±0.55)%。0.125μmol/L毒黄素组12、24和48 h细胞迁移率分别为7%、11%和16%,低于对照组相应的14%、26%和39%。结论毒黄素对A549细胞有明显增殖抑制和促凋亡作用,并呈时间和剂量依赖性,且可抑制A549细胞迁移活性。

Objective To investigate the inhibitory effects of toxoflavin on proliferation, apoptosis and migration of non-small cell lung cancer A549 cells. Methods The cultured A549 cells were treated with toxoflavin（0. 5, 0. 375, 0. 25, 0. 125 p, mol/L） for 24 h and 48 h, the control group was treated with ceil culture media only. CCK-8 assay was used to test the growth inhibitory rate and Annexin V/propidium iodide（PI） staining assay was applied to detect the apoptosis rate. The cell migration rate of 12 h, 24 h, 48 h were measured by the wound healing assay. Results The cell growth inhibition rates of A549 cells in experimental groups were （ 93.51 ±3.69）%, （40. 38±3.08）%, （23.54±2. 58）%, （ 13.07±2. 37）% in 24 h and（90. 53±3.58）%, （53.72±3.02）%, （34.44± 3.10） %, （24. 78±2.43）% in 48 h. The cell apoptosis rates of A549 cells in expremental groups were （78.68±2. 22）%, （43.66± 2.53）%,（20. 81±2.59）%,（6.25±0.96）% in 24 h and（88. 66±3.16）%, （59. 86±2. 81）%,（27.89±3.48）%,（9.91±1.33）% in 48 h, and those of control group were（ 1.57±0. 52）% in 24 h and （ 1.59±0. 55 ）% in 48 h respectively. The A549 cell migration rate of 12 h, 24 h ,48 h in experimental group（ 0. 125 p, mol/L） was 7%, 11% and 16% ）, but it was 14%, 26% and 39% in control group. Conclusion Toxoflavin could significantly inhibit proliferation and promote apoptosis of A549 cells in a dose and time depend- ent manner, and it could also weaken the migration capacity of A549 cells.