NtC3H基因对烟草类黄酮及绿原酸合成的影响

The Influence of Nt C3H on the Synthesis of Flavonoids and Chlorogenic Acid in Tobacco

为验证Nt C3H是否参与烟草中类黄酮和绿原酸等次生代谢物质的合成,构建了p CXSN::Nt C3H超量表达载体,利用农杆菌介导的叶盘转化法将Nt C3H基因转入野生型烟草三生烟,并通过HPLC检测转基因烟草中绿原酸以及芦丁、山奈酚芸香苷等类黄酮的含量变化。结果显示,与野生型三生烟草相比,转基因烟草叶片中绿原酸及芦丁的含量最高,分别提高了3.6倍和6.1倍,山奈酚芸香苷含量提高了24.6倍。研究结果证实了Nt C3H基因参与烟草中类黄酮和绿原酸等次生代谢物质的合成。

To test whether NtC3H participates in the biosynthesis of flavonoids, and chlorogenic acid (CGA) in tobacco and increases the contents of these secondary metabolites, we constructed the over-expression vector pCXSN::NtC3H and introduced it into Nicotiana tabacum var. samsun via Agrobacterium-mediated transformation. The concentrations of the different secondary metabolites including CGA, rutin, and kaempferol rutinoside in leaves of wild-type and transgenic lines were determined by HPLC. Compared to wild-type samsun tobacco, in transgenic tobacco the concentrations of CGA were increased 3.6-fold, rutin and kaempferol rutinoside were increased 6.1- and 24.6-fold respectively. These results suggested that NtC3H positively regulated the synthesis of flavonoids and CGA in tobacco.