RP-HPLC法测定米铂的含量

Determination of miriplatin by RP-HPLC

目的建立以反相高效液相色谱法测定米铂含量的方法。方法色谱柱为十八烷基硅烷键合硅胶柱(C_(18),4.6 mm×150 mm,5μm),流动相为叔丁醇-乙腈(9∶1),流速:1.0 m L·min^(-1),柱温:30℃,紫外检测波长:220 nm,进样量:20μL。结果米铂在40.68~305.10μg·m L^(-1)的检测浓度内呈良好的线性关系,R^2=1;平均回收率为100.13%(n=9),RSD为0.15%。结论本方法简便、快速、准确,回收率好,可用于米铂的含量测定。

Objective To establish a RP- HPLC method for the determination of miriplatin. Methods The ostade-cylsilane column( C_(18),4. 6 mm × 150 mm,5 μm) was used,the mobile phase was tertiary butyl alcohol- acetonitrile( 9∶1),the flow rate was 1. 0 m L·min^(-1),the column temperature was 30 ℃,the detection wavelength was 220 nm,the injection volume was 20 μL. Results A good linear was obtained in the range of 40. 68 ~ 305. 10 μg·m L^(-1)( R^2= 1). The average recovery was 100. 1%( n = 9),with RSD of 0. 15%. Conclusion The method was convenient,rapid,accurate and good recovery,it can be used for the determination of miriplatin.