8个紫花苜蓿品种的过氧化物同工酶分析

Analysis of Peroxidase Isozymes in Eight Alfalfa Varieties

利用非变性聚丙烯酰胺垂直板凝胶电泳技术,对8个野生紫花苜蓿品种过氧化物同工酶进行了分析,通过对各供试材料酶谱特征和相似系数的分析,探讨紫花苜蓿品种间的亲缘关系。结果表明,过氧化物同工酶在8个紫花苜蓿品种中均有表达,但各品种间的表达谱带及不同的同工酶表达量存有差异。8个品种共获得24条酶带,且存有1条共有表达酶条带,其中陇东野生紫花苜蓿同工酶谱带最多,表达有4条带;阿尔冈金酶谱带数最少,仅出现2条带。酶谱条带聚类分析表明,陇南野生紫花苜蓿与阿尔冈金间亲缘关系最远,亲缘关系呈现不同程度的差异。

Using native polyacrylamide gel electrophoresis, peroxidase isozymes of eight different varieties of alfalfa are analyzed. Based on the zymography characteristics of varieties tested, similarity coefficient and phylogenetic relationships among alfalfa varieties are obtained. The result shows that peroxidases in the varieties tested are expressed, however expression of all varieties varied. Total 24 peroxidase bands are observed and one major enzyme is presented. Moreover, expression level of the alfalfa cv. Longnan is predominately high and it has four enzyme bands. In contrast, expression level of alfalfa cv. Algonquin is low and possessed merely two enzyme bands. Analysis of zymography bands cluster shows that alfalfa cv. Longdong and alfalfa cv. Algonquin is the farthest in genetic distance. In addition, there are varying degrees of genetic differences between the other varieties.