吲哚胺2,3-双加氧酶及细胞因子在创伤后应激障碍患者中的表达变化及意义

Expression of Indoleamine 2,3-Dioxygenase and Cytokines in Patients with Post-traumatic Stress Disorder

目的探讨干扰素-γ(interferon-γ,INF-γ)、肿瘤坏死因子-α(tumor necrosis factor,TNF-α)、白介素-6(interleukin-6,IL-6)与吲哚胺2,3-双加氧酶(indoleamine 2,3-dioxy-genase,IDO)在创伤后应激障碍(PTSD)患者血清中表达变化及其临床意义。方法采集80例PTSD患者血清,同时收集80例健康对照组血清,运用酶联免疫吸附法(ELISA)和Western blot的方法对PTSD患者血清中INF-γ、TNF-α、IL-6及IDO进行检测,与对照组进行比较。结果实验表明PTSD组及正常对照组血清INF-γ、TNF-α、IL-6、IDO水平分别为[(28.43±11.23)vs.(3.01±1.12)pg/mL]、[(14.58±2.16)vs.(2.45±0.90)pg/mL]、[(16.34±5.31)vs.(2.01±1.08)pg/mL]、[(0.223 5±0.061 1)vs.(0.031 2±0.001 6)],PTSD组明显高于正常对照组(均P<0.01)。结论 INF-γ、TNF-α、IL-6及IDO表达的异常升高可能在PTSD的发病机制中扮演重要角色。

Objective To investigate the expression levels of interferon-γ（INF-γ）,tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）and indoleamine 2,3-dioxygenase（IDO）in the serum of patients with post-traumatic stress disorder（PTSD）and the clinical significance.Methods Serum samples were collected from 80 PTSD patients and 80 healthy individuals.The serum concentrations of INF-γ,TNF-α,IL-6and IDO were detected by enzyme-linked immunosorbent assay（ELISA）and Western blot in both groups.Results The concentrations of INF-γ,TNF-α,IL-6and IDO were（28.43±11.23）pg/mL,（14.58±2.16）pg/mL,（16.34±5.31）pg/mL and（0.223 5±0.061 1）,respectively,in PTSD group,significantly higher than those[（3.01±1.12）pg/mL,（2.45±0.90）pg/mL,（2.01±1.08）pg/mL and（0.031 2±0.001 6）]in control group（P〈0.01）.Conclusion Overexpression of INF-γ,TNF-α,IL-6and IDO may play an important role in the pathogenesis of PTSD.