微小RNA单核苷酸多态性与中国南方汉族人群变应性鼻炎易感性的研究

Study on singlecnucleotide polymorphisms of microRNA and susceptibility to allergic rhinitis in Han population of southern China

目的通过分析微小RNA（microRNA,miRNA）单核苷酸多态性（single-nucleotide polymorphisms,SNP）与变应性鼻炎易感性的关系,探索新的变应性鼻炎易感性分子标志物。方法选取216例变应性鼻炎患者设为变应性鼻炎组,另选取同期84名健康人群设为对照组,采集两组外周静脉血,提取基因组DNA,利用焦磷酸测序技术检测pre-miR-146a的SNP,分析其基因多态性位点rs2910164的基因型及等位基因频率。结果变应性鼻炎组pre-miR-146a（rs2910164）的CC基因型频率及等位基因C的频率显著高于对照组（P=0.031）。从基因型频率的相对风险分析发现,CC基因型患变应性鼻炎的风险增加（OR=1.799,95%CI：1.042~3.107）,C等位基因患变应性鼻炎的危险性也较G等位基因增加（OR=1.348,95%CI：1.036~1.754）。结论 pre-miR-146a（rs2910164）的SNP可能与中国汉族人群变应性鼻炎易感性相关,其突变位点可能是变应性鼻炎易感性的分子标志物。

Objective To explore the new molecular markers of susceptibility to allergic rhinitis by analyzing the rela- tionship between single nucleotide polymorphisms （SNP） of microRNA （miRNA） and susceptibility to allergic rhini- tis. Methods A total of 216 patients with allergic rhinitis were collected as the allergic rhinitis group, and 84 healthy people at the same term were collected as the control group. The peripheral venous blood was drawn in both groups, and genome DNA was extracted. The SNP of pre-miR-146a was detected using pyrosequencing technique to analyze its gene polymorphic site rs2910164 genotype and allele frequency. Results Both CC genotype frequency and allele C fre- quency of pre-miR-146a （rs2910164） in the allergic rhinitis group were dramatically higher than in the control group （P = 0. 031 ）. Through analysis on the relative risk of genotype frequency, it was found that the risk of patients with CC genotype who suffered from allergic rhinitis increased （OR = 1. 799, 95% CI： 1. 042-3. 107）, and the risk of those with allele C who suffered from allergic rhinitis also increased when compared with allele G （ OR = 1. 348, 95% C1： 1,036- 1. 754）. Conclusion The SNP of pre-miR-146a （rs2910164） may be associated with susceptibility to allergic rhinitis in Chinese Han population, and its mutation sites may be the molecular markers of susceptibility of allergic rhinitis.