解毒化瘀中药组方对免疫低下模型小鼠免疫功能的影响

Effects of detoxificating and stasis-removing herbal medicament on immune function in immunosuppressive mice

目的探讨解毒化瘀中药组方对免疫低下模型小鼠免疫功能的影响。方法采用地塞米松诱导小鼠免疫力低下模型,与正常对照组(无菌水)、阳性对照组(左旋咪唑)相比较,体内外检测不同剂量中药组方(体内剂量分别为2.4、4.8和7.2 g/kg,体外剂量分别为25、50和100 mg/L)对T、B淋巴细胞,NK细胞增殖程度及细胞活力的影响。淋巴细胞、NK细胞增殖率采用MTT法测定,NK细胞的杀伤活性以杀伤百分率表示。结果在体内,与正常对照组相比,无Con A诱导下,不同剂量中药组均可明显促进T淋巴细胞增殖(P<0.01);Con A诱导下,7.2 g/kg组使T淋巴细胞明显增殖(P<0.01);各剂量中药组方对LPS诱导的B淋巴细胞有增殖作用,2.4和4.8 g/kg中药组对NK细胞杀伤率有提高。在体外,在有或无Con A诱导下,50和100mg/L中药组使T淋巴细胞增殖,100 mg/L组可促进B淋巴细胞增殖(P<0.01);各剂量组对NK细胞增殖及杀伤率无明显影响。结论该中药组方可通过对T、B淋巴细胞的增殖及提高NK细胞的杀伤力来提高免疫力低下小鼠的免疫功能。

Objective To study the regulative effects of herbal medicament on the immune functions of immunosuppressive- model mice. Methods The dexamethasone induced immunosuppressive mice model was adopted. Comparison was made with the normal control group （sterile water） and the positive control group （levamisole）. In order to identify the role of the immune function of differ- ent doses of the herbal medicament on proliferation degree of T and B lymphocyte as well as the influence on the NK cells activity in vivo and in vitro were tested. The low, medium and high dose were 2.4, 4.8 and 7.2 g/kg respectively in vivo, and 25, 50 and 100 mg/L respectively in vitro. Results The herbal medicament had obvious proliferation effect on T lymphocyte of immunosuppressive mice model induced by dexamethasone with or without ConA stimulation in vivo and in vitro （P〈0.01）. Proliferation function was also shown on B lymphocyte of mice with or without stimulation of LPS in vivo and in vitro （P〈0.01）. It had no proliferation effect on NK cells in vivo and in vitro. Under the condition with or without interleukin stimulating NK cell killing rate was all increased. Conclusion The herbal medicament improves the immune function of low immunity mice mainly based on proliferation of T and B lymphocytes and increased lethality of NK cells.