利用SSR标记鉴定花生杂交F1代真假杂种

Identification of Peanut F_1 Hybrids with SSR Markers

以抗青枯病花生品种日花1号、山东省花生研究所选育的高油酸花生品种花育662和高产大花生品种花育9610为亲本正、反交获得的F_1代种子为材料,利用微卫星(SSR)标记技术鉴定真杂种;从候选的50对SSR引物中筛选得到在亲本中有稳定、清晰多态性位点的引物5对,对杂种F_1代进行鉴定。结果表明,四个杂交组合共鉴定出30粒真杂种,并得到形态学鉴定结果的确认。

To identify the F1 true hybrids of four peanut cross combinations, SSR molecular markers were employed. The parents of those combinations were Ri Hua 1, a peanut cuhivar with high resistance to bacterial wilt （BW）, HY662, a high oleate peanut variety, and HY9610, a high yielding variety. Five polymorphic SSR primer pairs of fifty SSR primer pairs were selected to distinguish the true hybrids. Thirty true F1 hybrid individuals were identified and got confirmation of morphological identification.