野火球煎剂对小鼠镇静催眠作用的影响

The Sedative and Hypnotic Effects of Trifolium lupinaster Decoction on Mice

[目的]研究野火球煎剂的镇静催眠作用。[方法]试验分为空白对照(等容生理盐水)、地西泮(0.001 g/kg)与野火球煎剂高、中、低剂量(3.12、1.56、0.78 g/kg)组,通过小鼠自主活动试验观察其对小鼠活动的影响,通过阈上和阈下剂量戊巴比妥钠的睡眠试验观察其对小鼠入睡潜伏期和睡眠时间的协同影响和尼可刹米对小鼠的惊厥作用。[结果]与空白对照组比较,野火球煎剂高、中、低剂量组小鼠自主活动次数显著减少,可显著缩短小鼠睡眠潜伏期、延长小鼠睡眠时间,且野火球煎剂有抗尼可刹米致惊厥发作的作用。[结论]野火球煎剂具有镇静催眠作用和抗惊厥作用,为进一步研究野火球镇静催眠作用机制奠定基础。

[ Objective ] To research the sedative and hypnotic effects of Trifolium lupinaster decoction. [ Method ] Mice were randomly divided into five groups, which were blank control （normal saline）, diazepam group （0.001 g/kg）, high-, middle-and low-dosage groups （3.12, 1.56, 0.78 g/kg） of T. lupinaster decoction. Mice activity was studied by spontaneous activity experiment. Sleep test of pentobarbital sodium at suprali minal and subliminal doses was carried out to observe the convulsion effects of nikethamideon mice, as well as the collaborative effects on sleep latency and sleeping time of mice. [ Result] Compared with blank control group, autonomic activity times of mice reduced significantly at high-, middle- and low-dosage groups of T. lupinaster. Thus, the sleep latency of mice decreased significantly; and the sleeping time of mice prolonged. T. lupinaster decoction had the anti-seizures effects induced by nikethamide. [ Conclusion] T. lupinaster decoction has both anticon- vulsant effects and sedative hypnotic effects, which lays foundation for the further research on sedative hypnotic effects of T. lupinaster.