马钱苷对糖基化终末产物诱导足细胞损伤的保护作用

Protective effect of loganin on podocyte injury induced by advanced glycation end products

目的探讨山茱萸环烯醚萜苷特征性成分马钱苷对糖基化终末产物(AGEs)诱导肾脏足细胞损伤的保护作用及其机制。方法体外培养小鼠肾小球足细胞,分为空白对照组、模型组(AGEs组)、马钱苷组,并设氨基胍组作为阳性对照。MTT法检测马钱苷对足细胞存活率的影响;Hoechst33342/PI双染观察足细胞凋亡情况,流式细胞仪检测细胞凋亡率;Western blot法检测足细胞AGEs受体(RAGE)、足细胞损伤标志蛋白desmin以及凋亡相关蛋白bax、bcl-2、cleaved caspase-3的表达。结果马钱苷能够抑制AGEs导致的足细胞损伤,下调足细胞Desmin、RAGE蛋白的表达,明显降低AGEs诱导的足细胞凋亡率,降低足细胞Bax/Bcl-2的比值和促凋亡蛋白cleaved caspase-3的表达。结论马钱苷能够改善AGEs诱导的肾小球足细胞损伤,其作用机制与降低RAGE蛋白表达,抑制凋亡通路有关。

Aim To explore the protective effect of loganin(an active component in Cornus officinalis) on podocyte injury induced by advanced glycation end products(AGEs) and its possible mechanism.Methods Mouse podocytes were cultured in vitro and divided into Normal group,model group(AGEs group),loganin group and aminoguanidine group( set as positive control).After being incubated with loganin( final concentrations are 0.1,1,10 μmol·L^(-1)) for 1 h,podocytes were stimulated by AGEs of 100 mg·L^(-1) for 24 h.Then,the cell viability was measured by using MTT method.Podocytes apoptosis was evaluated by Hoechst33342/PI staining and flow cytometry.Receptors of advanced glycation end products(RAGE),desmin and apoptosis-related protein like Bax,Bcl-2,cleaved caspase-3 in podocytes were detected by Western blot.Results Loganin ameliorated podocyte injury induced by AGEs,down-regulated the expression of desmin and RAGE.Loganin also reduced the apoptotic rate of podocytes and decreased the ratio of Bax/Bcl-2and the expression of pro-apoptotic protein cleaved caspase-3 in podocytes.Conclusion Loganin could ameliorate podocyte injury,and its mechanism may be related to the decrease of the expression of RAGE and inhibition of the apoptotic pathway.