浙产女贞子中红景天苷含量动态变化及区域差异性研究

Study on Dynamic Changes and Regional Differences of Content of Salidroside in Ligustri Lucidi Fructus in Zhejiang Province

目的 采用高效液相色谱法测定不同生长期、不同区域浙产女贞子中的红景天苷含量,为更合理、有效地从女贞子中提取红景天苷提供依据.方法 色谱柱：Kromasil C18（4.6 mm×150 mm, 5 μm）;流动相：乙腈-水（10∶90）;流速：1.0 mL/min;检测波长：220 nm;柱温：室温;进样量：20 μL.结果 红景天苷在0.71～3.55 μg 范围内与峰面积积分值呈良好线性关系（r=0.999 4）;加样回收率在95.6%～98.6%之间,平均加样回收率为96.88%,RSD=1.32%（n=5）.结论 红景天苷在女贞子中的含量随生长期延长逐渐下降,不同区域、不同采收期女贞子中红景天苷的含量不同.

Objective To determine content of Salidroside in Ligustri Lucidi Fructus at different growth stages and in different regions in Zhejiang Province by HPLC; To provide the basis for more rational, efficient extraction of Salidroside from Ligustri Lucidi Fructus.Methods The Kromasil C18 column (4.6 mm×150 mm, 5μm) was used with mobile phase of acetonitrile-water (10:90); flow rate was 1.0 mL/min; detection wavelength was 220 nm; column temperature was set at room temperature; injection volume was 20μL.Results Salidroside was in good linear range within 0.71–3.55μg (r=0.999 4). The recovery rate of Salidroside was 95.6%–98.6% and the average recovery rate was 96.88%, RSD was 1.32% (n=5).Conclusion The content of Salidroside in Ligustri Lucidi Fructus gradually decreased with the extending of growing season. The contents of Salidroside were different at different growth stages and in different regions.