CA125、Cyt-c和MGMT基因在卵巢癌患者血清中的表达及临床意义

The expression and clinical value of serumm carbonhydrate antigen 125,Cytochromes c and MGMT in patients with ovarian cancer

目的:探讨糖类抗原125(carbohydrate antigen 125,CA125)联合细胞色素C(Cytochromes c,Cyt-c)和O^6-甲基鸟嘌呤-DNA-甲基转移酶(MGMT)在卵巢癌患者血清中的表达情况及临床意义。方法:对53例卵巢癌患者(卵巢癌组)、40例卵巢良性病变患者(卵巢良性病变组)、50例同期体检健康女性(对照组)采用电化学发光法检测血清CA125水平,ELISA法检测Cyt-c水平,甲基化特异PCR法检测MGMT基因启动子甲基化,流式细胞仪检测细胞线粒体膜势能。结果:卵巢癌患者血清中CA125和Cyt-c表达水平明显高于卵巢良性肿瘤组和健康对照组(P<0.05),CA125表达水平在卵巢良性肿瘤组和健康对照组,差异无统计学意义(P>0.05),Cyt-c差异有统计学意义(P<0.05)。MGMT基因启动子在卵巢癌组和卵巢良性肿瘤组中发生甲基化,甲基化率分别为75.3%和21.8%,健康对照组未发生甲基化。结论:联合检测血清CA125、Cyt-c和MGMT基因的表达水平有助于提高早期卵巢癌的诊断效能,对卵巢肿瘤的良恶性鉴别及对预后具有提示作用。

Objective： To investigate the expression of CA125,Cyt-c and O^6-methylguanine DNA methyltransferase（ MGMT） and its clinical significance in cervical cancer. Methods： Methylation-specific PCR was used to detect the MGMT methylation status,mitochondrial Transmembrane potentials were examined using flow cytometric analysis. The serum level of CA125 was detected by electro-chemiluminescence,the level of Cyt-c was detected by ELISA respectively in 53 patients with ovarian cancer（ cancer group）,40 patients with benign ovarian tumor（ benign group） and 50 healthy females（ control group）. Results： The level of CA125 and MGMT methylation status was significantly higher in cancer group than those in benign group and control group,the positive rates were 75. 3% and21. 8% respectively（ P〈0. 05）. Cyt-c was lower in cancer group than those in benign group and control group.Conclusion： The combined detection of CA125,Cyt-c and MGMT methylation status contributes to improving the diagnosis efficiency,and has a certain application value to the differential diagnosis of benign and malignant ovarian tumor.