冠状动脉结扎制备大鼠心肌梗死模型及评价实验研究

Establishment and evaluation experimental study of acute myocardial infarction in rats

[目的]探讨建立稳定可靠、易操作和高存活率的大鼠心肌梗死模型制备方法及其有效的评价手段。[方法]40只雄性SD大鼠腹腔麻醉后,开胸手术结扎其冠状动脉左前降支。术后24 h,超声心动图观察左室功能变化,左室导管术检测大鼠血流动力学,取出心脏,观察心脏外形,切片进行2,3,5-氯化三苯基四氮唑(TTC)染色,全自动生化仪检测血液心肌酶学指标。[结果]该法的手术存活率高达91.67%,术后24 h取出心脏可见左心室梗死区心室壁颜色较周围心肌浅,超声心动图和血流动力学结果显示模型组大鼠左室功能及室壁运动情况明显弱于假手术组。TTC染色显示模型组大鼠心肌梗死明显。[结论]该方法提高了大鼠急性心肌梗死模型的存活率,模型稳定,重复性好,可满足一次结扎或缺血再灌注的需求。

[Objective] To explore the establishment of a stable and reliable, easy operation and high survival rates of rats with acute myocardial infarction model preparation and effective evaluation methods. [Methods] The 50 male SD rats were anesthetized,thoracotomy left anterior descending coronary artery ligation. After 24 h, observed with echocardiograph changes in left ventricular function and left ventricular catheterization rat blood dynamics. Remove the heart, TTC sections were stained; automatic biochemical analyzer to detect blood serum creatine kinase indicators. [Results] This method of surgery survival rates as high as 91.67%. After 24 h,remove the cardiac left ventricle infarcted ventricular wall visible color of light than the surrounding myocardium. In the model group,echocardiographic and hemodynamic showed left ventricular function and wall motion was obviously weaker than the sham group. TTC staining showed significant myocardial infarction in the model group. [Conclusion] This method improves the survival rate of rats with acute myocardial infarction model, the model is stable, reproducible, and can meet once ligation or ischemia-reperfusion needs.