淫羊藿化学成分分离鉴定

Isolation and identification of chemical constituents from Epimedium brevicornu Maxim

[目的]研究淫羊藿叶的化学成分。[方法]综合运用溶剂法提取、萃取、正相硅胶、Sephadex LH-20、反相ODS以及制备高效液相色谱等分离纯化方法,对淫羊藿叶化学成分进行分离,通过质谱、核磁共振波谱等方法鉴定各单体化合物结构。[结果]从淫羊藿干燥叶的95%乙醇提取物（V/V）中共分离鉴定7个化合物,分别为2,3,4,6,7-五甲氧基-菲（1）、4,5,-二羟基-2,3,6-三甲氧基-9,10-二氢菲（2）、5,7,4′-三甲氧基-4-苯基香豆素（3）、3,5,7,4′-四甲氧基黄酮（4）、4-羟基-3-异戊二烯基-苯甲酸（5）、4-羟基肉桂酸甲酯（6）、4-羟基-（2E）-2-壬烯酸（7）。[结论]化合物1～7均为首次从淫羊藿属分离得到化合物。

[Objective] To investigate chemical constituents from the leave of Epimedium brevicornu Maxim. [Methods] The compounds were purified by solvents extracting and several column chromatography, including silica gel, Sephadex LH-20, ODS and preparative HPLC. Their structures were identified by MS and NMR spectra. [Results] Seven compounds were separated from 95% ethanol（V/V）extract of E. brevicornu and identified as 2,3,5,6,7-pentamethoxy-phenanthrene（1）, 4,5-dihydroxy-2,3,6-trimethoxy-9,10-dihydrophenanthrene（2）, 5,7,4′-trimethoxy-4-phenylcoumarin（3）, 3,5,7,4′-tetramethoxyflavone（4）, 4-hydroxy-3-prenylbenzoic acid（5）, 4-Hydroxycinnamic acid methyl ester（6）, and（2E）-2-nonenoic acid, 4-hydroxy（7）. [Conclusion] Compound 1 ～7 were obtained from genus of Epimedium for the first time.