PDGF及其基因多态性与冠状动脉粥样斑块易感相关性研究

Association among PDGF and its gene polymorphism with coronary artery atheromatous plaque

目的:探讨血小板衍化生长因子(PDGF)血清学水平及PDGF-D rs3809021、rs974819位点基因多态性与冠状动脉粥样硬化斑块性质遗传易感性的关系。方法:237例冠心病(CHD)患者根据冠脉造影及血管内超声检查(IVUS)结果将粥样斑块分为稳定斑块组(65例)、易损斑块组(89例)、混合斑块组(83例),采用双抗体夹心酶联免疫吸附法(ELISA法)检测血清PDGF水平;应用聚合酶链反应(PCR)技术检测PDGF-D rs3809021、rs974819基因多态性及测序鉴定,并与112例正常者对照。结果:CHD各亚组PDGF水平均高于对照组;易损斑块组、混合斑块组血清PDGF水平均显著高于稳定斑块组。PDGF-D基因rs3809021位点多态性在CHD组和对照组的分布差异无统计学意义。PDGF-D基因rs974819位点中,携带C等位基因的基因型(CG+CC)可增加总体CHD发病风险,CHD亚组间基因频率分布特点为稳定斑块组与易损斑块组、混合斑块组相比较,差异有统计学意义。结论:PDGF-D rs974819位点基因多态性与冠状动脉粥样硬化斑块性质的相关,C等位基因使易损斑块的发生率增加,rs974819位点C等位基因可能是CHD的遗传易感基因。

Objective：To investigate whether platelets-derived growth factor（PDGF）and its gene polymorphism is associated with coronary atherosclerotic plaque.Method：According to the morphological division of the plaque,patients examined with coronary angiography and intravenous ultrasound were divided into three groups of stable plaque group（65 cases）,vulnerable plaque group（89 cases）and mixed plaque group（83 cases）.The gene polymorphism was measured by the polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP）and identified by sequencing.Double antibody sandwich enzyme-linked immunosorbent（ELISA）was used to detect PDGF level in serum.Result：All subgroups of coronary heart disease（CHD）had higher PDGF level than the control group.Furthermore,PDGF level in vulnerable plaque and mixed plaque group was significantly higher than that in the stable plaque group.The distribution of PDGF-D gene rs3809021 polymorphism was not significant different between CHD group and control group.Carrying C allele genotype（CG＋CC）of PDGF-D gene rs974819 could increase the overall risk of CHD.The gene frequency distribution characteristics in the stable plaque group were significant different from vulnerable plaque and mixed plaque group.Conclusion：PDGF-D rs974819 polymorphism is associated with the nature of the coronary atherosclerotic plaque.Callele increase the incidence of vulnerable plaques.rs974819 allele is an important genetic susceptibility gene for CHD.