芪叶保肝饮对大鼠肝脏纤维化及星形细胞活化的影响

Effects of Liver-protecting drinking from Stilbene leaf on the liver fibrotic and activation of hepatic stellate cells in rats

目的通过研究芪叶保肝饮(Liver-protecting drinking from Stilbene leaf,SPDS)对大鼠肝脏纤维化及星形细胞活化的影响,探讨SPDS抗肝纤维化的作用机制。方法 30只SD大鼠,随机分为对照组、肝纤维化组、SPDS保护组。采用CCl4复制大鼠肝纤维化模型,肝纤维化组和SPDS保护组均为肝纤维化模型大鼠。SPDS组建模同时,以15 m L/kg的剂量标准灌服SPDS溶液,每日2次,持续至第8周。处死小鼠去血清和肝组织,检测血清中肝纤维化指标:透明质酸(HA)、Ⅳ型胶原(CⅣ)、层粘连蛋白(LN),抗氧化指标:肝匀浆丙二醛(MDA)、超氧化物歧化酶(SOD)。肝组织病理切片HE染色观察组织形态变化,免疫组织化学方法检测α-平滑肌肌动蛋白(α-SMA)的表达。结果与肝纤维化组相比,SPDS可以明显减少HA、CⅣ、LN等肝纤维化相关指标的升高程度(P<0.01);显著提高了过氧化物歧化酶活力(P<0.01),降低了MDA含量(P<0.01);同时也抑制了肝星形细胞对α-SMA的表达(P<0.01),显著降低了肝纤维化的病理学分期(P<0.05)。结论 SPDS可能通过抗氧化作用,减轻肝脏损伤,抑制星形细胞的活化,从而减轻CCl4诱导的肝脏纤维化。

Objective To study the effects of Liver- protecting drinking from Stilbene leaf（ SPDS） on the liver fibrotic and activation of hepatic stellate cells in rats,and then investigate the anti- fibrotic mechanism of SPDS. Methods Liver fibrosis model was induced in rats by CCl4 administration,and was treated with SPDS. The expression of hepatic stellate cells positive for α-smooth muscle actin（ α- SMA）,the collagen synthesis and other indicators were studied at the end of the experiment. Results Compared with liver fibrosis model group,SPDS can significantly reduce hyaluronic acid,collagen Ⅳ,laminin,and other relevant indicators of liver fibrosis（ P〈0. 01）; effectively improve the vitality of superoxide dismutase（ P〈0. 01）; significantly reduce serum levels of ALT and inflammatory cytokines TGE- β（ P〈0. 01）; and also suppress the expression of α- SMA in hepatic stellate cells in liver（ P〈0. 01）. Conclusion The anti- oxidation effects of SPDS can alleviate liver damage and inflammation,inhibit the activation of stellate cells,and then reduce CCl4- induced liver fibrosis in rats.