戊型肝炎病毒在长爪沙鼠体内消长规律研究

Study on Viral Dynamics in Gerbils Experimentally Infected with Hepatitis E Virus

为掌握戊型肝炎病毒(HEV)感染长爪沙鼠后各组织器官中病毒的载量及消长规律,以1株云南猪源HEV感染ZCLA长爪沙鼠,采用实时荧光定量PCR相对定量法,对不同感染时间的血液、粪便及肝、肠等组织中的病毒进行测定。结果表明,从感染3d后开始,长爪沙鼠血液、粪便、肝、肠和胆汁中均能检测到病毒,以肠和胆汁中含量最高,感染5周后各组织中还能检测出病毒。研究结果为建立长爪沙鼠感染HEV模型,揭示HEV复制与疾病进程的关系、致病机理,以及建立防控技术等奠定基础。

In order to determine the viral dynamics during infection,in this study ZCLA Mongolia gerbils were experimentally infected with a genotype 4strain isolated from Yunnan pig farms,and viral loads of HEV in blood,feces,livers and intestine tissues at different infection stages were determined by using realtime PCR.The results showed that HEV could be detected in all samples since 3days post infection,high titers were found in intestines and biles.These results lay agood foundation for establishment of the HEV infection model,and further study of the correlation between the viral replication and the progress of disease,pathogenesis of hepatitis,prevention and control.