副猪嗜血杆菌HigB和HigA基因的原核表达及功能分析

Prokaryotic Expression and Function Analysis of Haemophilus parasuis HigB and HigA Genes

副猪嗜血杆菌属于条件性致病菌,可引起猪的格拉泽病,表现为多发性浆膜炎和关节炎。毒素抗毒素系统(TA)广泛存在于细菌中,其参与各种应激状态下的生理调节,利于机体适应复杂环境。克隆编码副猪嗜血杆菌的HigBA毒素抗毒素蛋白的基因,并对其进行原核表达和分析鉴定。结果发现,HigB对生长有抑制作用,而HigA可以中和这种作用,HigBA构成一对毒素抗毒素系统。HigB在高温和高密度时,表达量显著增加,显示其参与逆境环境下的生理调控。

Haemophilus parasuis is a conditional pathogenic bacterium,and could cause swine Glaser＇s disease,which is manifested as polyserositis and arthritis.TA system widely exists in bacteria,which involves in physiological regulation in stress states for adapting to complex environment.There are few reports about the TA system of Haemophilus parasuis,so the genes of HigB and HigA were screened and cloned,and then they were expressed and identified in prokaryotic expression system.The results showed that,HigBA is a pair of toxin-antitoxin system,in which HigB could inhibit the growth,while HigA could neutralize this effect.The expression of HigB was significantly increased at high temperature and high density,which indicated that it participated in the physiological regulation of stress.