SB225002与顺铂联合应用对口腔鳞癌细胞作用的体外研究

Effects of Combined Chemotherapy of SB225002 and Cisplatin on OSCC Cells In Vitro

目的:观察SB225002与顺铂联合应用对口腔鳞癌细胞的增殖、凋亡及细胞周期的影响,并探讨其机制。方法:分别采用MTT试验及流式细胞术检测SB225002与顺铂单独或联合应用对体外培养的口腔鳞癌细胞的增殖、凋亡及细胞周期的影响,并进行相关分析。结果:在单独应用时,SB225002可一定浓度范围内显著抑制口腔鳞癌细胞的增殖,促进其凋亡,并诱导细胞发生G2/M期阻滞,作用随浓度升高而增强。SB225002与顺铂联合应用时,联合组对口腔鳞癌细胞的抑制效果明显强于单独用药组,P<0.05。在一定浓度范围内,SB225002可显著促进口腔鳞癌细胞的凋亡,并诱导细胞发生G2/M期阻滞,其作用随浓度的升高而增强;而SB225002与顺铂联合应用时,联合组的促调亡及诱导细胞G2/M期阻滞作用均显著强于单独用药组,P<0.05。结论:SB225002单独或与顺铂联合应用时,均可显著抑制口腔鳞癌细胞的增殖,促进其凋亡,并诱导细胞发生G2/M期阻滞,两者之间存在协同作用。

Objective： To investigate the effects of SB225002 and cisplatin on oral squamous cell carcinoma （OS- CC） ceils, and meanwhile explore the possible mechanism. Methods： The OSCC cell proliferation, apoptosis and cell cycle were detected by MTT assay and flow cytometry after treated with SB225002 and cisplatin alone or in combination, and then correlated analysis was performed. Results： SB225002 could significantly inhibit the prolifer- ation, promote apoptosis and induce G2/M arrest of OSCC cells in a concentration--dependent manner. Moreover, combined chemotherapy of SB225002 and cisplatin showed stronger effects, and the differences were statistically significant （P〈0.05）. Conclusion： There is synergistic effect between SB225002 and cisplatin on the anti--tumor activity in OSCC cells.