摘要
目的:研究补肾健脾活血解毒煎剂对单侧输尿管梗阻大鼠肾间质α-平滑肌肌动蛋白(α-SMA)、转化生长因子-β1(TGF-β1)表达的影响,探讨其抗肾间质纤维化的作用机制。方法:建立大鼠单侧输尿管梗阻(UUO)模型,50只SD大鼠随机分为假手术组、模型组、代文组、补肾健脾活血解毒煎剂低、中剂量组,于造模4周后腹主动脉采血检测血清肌酐(Scr)、尿素氮(BUN),收集24 h尿液检测N-乙酰-β-D-氨基葡萄糖苷酶(N-acetyl-β-D-glucosaminidase,NAG)、β2-微球蛋白(β2-microglobulin,β2-MG),取梗阻侧肾组织,HE、Mason染色观察肾小管间质病变,免疫组织化学染色观察α-SMA、TGF-β1在肾间质的阳性染色表达,并进行半定量分析。结果:与假手术组比较,模型组大鼠Scr、BUN、尿NAG、β2-MG水平以及α-SMA、TGF-β1阳性表达显著升高(P<0.01);与模型组比较,补肾健脾活血解毒煎剂低、中剂量组大鼠Scr、BUN水平降低,尿NAG、β2-MG排泄减少(P<0.05或P<0.01),与代文组相当;补肾健脾活血解毒煎剂低、中剂量组和代文组α-SMA、TGF-β1阳性表达均显著下调(P<0.01)。结论:补肾健脾活血解毒煎剂可能通过改善肾小球、肾小管功能,抑制α-SMA、TGF-β1的表达,从而抑制肾小管上皮细胞间充质转分化,达到改善肾间质纤维化的作用。
Objective: To investigate the effects of BSHXJDJJ on the renal interstitium alpha-smoothmuscle actin(α-SMA) and transforming growthfactor-betal (TGF-β1) expression in unilateral ureteral obstruction (UUO)rats, and to illuminate the possible mechanisms against renal interstitial fibrosis. Methods: The rat modle with unilateral ureteral obstruction (UUO) was created. The 50 Sprague-Dawley rats were randomly divided into Sham-op- erated, modle, Diovan, and the low and middle dose BSHXJDJJ groups. On the 4th week after treatment, the serum creatinine(Scr), blood urea nitrogen(BUN), the N-acetyl-β-D-glucosaminidase(NAG), andβ2-microglobuliu (β2-MG)in 24 h urine were detected; the obstructed kidney was taken outand measured by HE, Masson, and immunohistochemistry staining for semiquantitative analysis of a-SMA and TGF-β1. Results: The level of Scr, BUN, NAG,β2-MG,α-SMA and TGF-β1 in model group increased (P〈0.01), compared with those in the sham-ope rated group. In comparison with the model group, the lever of Ser, BUN, NAG and β2-MG were significantly lower in the low and middle dose BSHXJDJJ groups (P〈0.05 or P〈0.01);and the expression of positive staining for α-SMA and TGF-β1 were significantly down-regulated in the low and middle dose BSHXJDJJ groups and Diovan group (P〈0.01). Conclusion: The BSHXJDJJ can degrade renal interstitial fibrosis by improving renal function, inhibiting the expression of a-SMA and TGF-β1, and inhibiting renaltubule epithelial-mesenchymal transition.
出处
《中医药导报》
2016年第5期10-15,共6页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
江苏省中医药局课题(LZ13074)
关键词
肾间质纤维化
补肾健脾活血解毒煎剂
单侧输尿管梗阻
α-平滑肌肌动蛋白
转化生长因子-β1
renal interstitial fibrosis
Bushen Huoxue Jiedu Jianji (初肾健脾活血解毒煎剂, BSHXJDJJ)
unilateral ureteral obstruction
Alpha-Smooth muscle acfin
transforming growth factor-betal