背部循经推拿手法对束缚应激模型大鼠亚健康样行为及HPA轴相关激素水平的影响

The Effect of Chinese Massaging along the Meridians on the Back on Sub-Health Sample Behavior and HPA Axis Related Hormone Levels in Restraint Stress Model Rats

目的:采用实验方法观察推拿对应激大鼠亚健康样行为和HPA轴相关激素水平的影响。方法:将60只wistar大鼠随机分为正常组、模型组和推拿组,后两组予束缚应激,推拿组予背部循经推拿。实验前1 d及第7、14、21天利用鼠尾悬挂试验,实验前1 d及第21天利用旷场试验,分别观察3组大鼠的行为学改变。实验结束后放免法测血清促肾上腺皮质激素(ACTH)、皮质酮(CORT)水平。结果:旷场试验结果:实验结束后,模型组大鼠中央格停留时间明显增加,跨格次数、修饰次数及修饰时间减少,与实验前组内比较,差异有统计学意义(P<0.05);与正常组比较,差异有统计学意义(P<0.05)。与实验后模型组比较,推拿组的中央格停留时间减少,跨格次数、修饰次数及修饰时间增加,差异有统计学意义(P<0.05)。鼠尾悬挂试验结果:实验结束后模型组大鼠不动时间明显增加,挣扎次数明显减少,与实验前比较,差异有统计学意义(P<0.05);实验结束后模型组挣扎次数明显少于正常组(P<0.05),不动时间明显多于正常组(P<0.05);随着造模时间的延长,大鼠悬尾试验不动时间增加、挣扎次数减少,而推拿组不动时间则显著短于同期模型组(P<0.05),挣扎次数则多于同期模型组(P<0.05)。3组大鼠实验结束后血清CORT、ACTH水平检测结果:模型组大鼠血清CORT浓度明显高于正常组,差异有统计学意义(P<0.05);推拿组大鼠血清CORT浓度明显低于模型组,差异有统计学意义(P<0.05)。模型组、推拿组血清ACTH浓度明显高于正常组,差异均有统计学意义(P<0.05);推拿组血清ACTH浓度明显低于模型组,差异有统计学意义(P<0.05)。结论:背部循经推拿手法能改善应激模型大鼠的躯体与精神疲劳状态,可能作用机制为调节HPA轴异常的功能活动。

Objective： To evaluated the effect of Chinese massaging along the meridians on the back on sub-health sample behavior and HPA axis related hormone levels in stress model rats, by experimental method. Methods： The 60 Wister rats were divided into normal feeding group, model group and Chinese massag group. The latter two groups were treated with restraint stress, beisdes, and the massage group with massage. Before the experiment and 1st, 7th, 14th and 21th day during the experiment, the rat pathological changes in 3 groups were observed by using rat tail suspension test and open field test. And 1st and 21th day after the experiment, the serum levels of ACTH and corticosterone （CORT） were measured by radioimmunoassay. Results： Open tank test showed that at the end of the experiment, the central cell residence time increased significantly and the times of crossing grids, and the modification times and modification time reduced in the model group, with significant difference （P〈0.05）; Compared with the normal group, differences are also significant （P〈0.05）. After the experiment, the massage group showed less central cell residence time and more the times of crossing grids, and the modification times and modification time than the model group, with significant difference （P〈0.05）; Rat tail suspension test results showed that at the end of the experiment, immobility time increased significantly, andthat the struggling times reduced significantly in the model group, with significant difference （P〈0.05）; at the end of the experiment, the model group showed less struggling times and more immobility time than the normal group （P〈0.05）; with the extension of the modeling time, the duration of immobility increased and struggling number reduced, and the massage group showed less immobility time than the model group at the same period （P〈O.05）, with more struggling times than model group （P〈0.05）. The serum CORT and ACTH levels results showed that model group showed significantly higher than serum CORT concentration than normal group, and that the difference was significant （P〈0.05）; massage group showed significantly lower serum CORT concentration than the model group, and the difference is significant （P〈0.05）. the model group and massage group showed higher ACTH levels than the norml group, with significant difference （P〈0.05）; The massage group showed lower higher ACTH levels than model group, with significant difference （P〈0.05）. Conclusion： Chinese massaging along the meridians on the back can improve physical and mental fatigue in stress model rats, and the modulating effect is realized by adjusting the HPA axis function.