瘢痕疙瘩中成纤维细胞耐药基因mdr1的研究

Research of Drug Resistance Gene MDR1 in Keloid Fibroblasts

目的研究瘢痕疙瘩成纤维细胞(KF)与正常皮肤组织成纤维细胞(NF)耐药基因mdr1的表达差异。方法利用RT-PCR、免疫组化及流式细胞分析等方法,检测瘢痕疙瘩与正常皮肤组织中成纤维细胞耐药基因mdr1及其蛋白ABCB1的表达;并对30例不同部位瘢痕疙瘩的ABCB1蛋白表达量进行流式细胞分析。结果瘢痕疙瘩成纤维细胞中耐药基因mdr1及其蛋白ABCB1的表达明显高于正常皮肤;流式细胞分析显示,瘢痕疙瘩原代成纤维细胞中ABCB1表达率:胸部>背部、腹部,差异有统计学意义。结论瘢痕疙瘩成纤维细胞表达耐药基因mdr1及其蛋白ABCB1,且较正常皮肤中的表达量增高。

Objective To compare the expression of drug resistance gene mdr1 between keloid fibroblast（KF） and normal dermal fibroblast（NF）. Methods The expression of drug resistance gene mdr1 and its corresponding protein ABCB1 in KF and NF were assessed with real-time PCR, immunohistochemical and flow cytometry. The expression of ABCB1 protein in 30 cases of keloid was analyzed by flow cytometry. Results The expression of mdr1 and ABCB1 in keloid was significantly higher than in normal skin; The expression rate of ABCB1 of the primary fibroblasts in pectoral keloid was higher than in abdominal and dorsal keloid. Conclusion Drug resistance gene mdr1 and its corresponding protein ABCB1 are all expressed in KF, and the expression amount is much higher than in NF.