摘要
以碱性蛋白酶水解白鲢鱼肉,采用超滤杯做酶反应器来实现可控酶解白鲢鱼肉,研究超滤膜的型号和操作压力对酶解产物的氮收率、水解度、平均肽链长度和分子量分布的影响。结果表明:选用截留分子量为10KD的超滤膜,在0.15MPa下边超滤边酶解,滤液的平均肽链长度为3.59,水解度为11.92%,氮收率为39.2%,分子量分布是连续的,且较集中。
The alcalase was selected for hydrolysis of silver carp protein, ultrafitration（UF） cup was used as a controllable reactor to enzymatic hydrolysis, and effects of membrane type and operation pressure on the nitrogen yield,degree of hydrolysis, average length of peptide chain, molecular weight distribution were studied. Results showed that ultrafiltrating at operational pressure 0.15 MPa with 10 k D membrane at the optimum enzymatic conditions, the average length of peptide chain, degree of hydrolysis and nitrogen yield of the permeate were 3.59,11.92% and 39.2%,respectively. And the molecular weight distribution of the permeate is continuous and more concentrated.
出处
《食品与发酵科技》
CAS
2016年第1期43-46,59,共5页
Food and Fermentation Science & Technology
关键词
白鲢
超滤
酶水解
分子量分布
Silver Carp
ultrafiltration
enzyme hydrolysis
molecular weight distribution
