羧甲基壳聚糖包覆薏苡仁油前体脂质体的稳定性研究

Study on the stability of carboxymethylchitosan- coated proliposomes loaded coix seed oil

为评价羧甲基壳聚糖包覆薏苡仁油前体脂质体(CM-PL)的贮藏稳定性及复水稳定性,本研究以贮藏过程中薏苡仁油(CSO)渗漏率、丙二醛(MDA)生成量为主要指标系统考察了CM-PL在不同贮藏条件下的贮藏稳定性;以CSO渗漏率为指标考察了氯化钠溶液、葡萄糖溶液对CM-PL复水稳定性的影响。结果表明:光照、高温以及空气接触均影响CM-PL的稳定性,其影响顺序为温度>空气>光照;其中温度升高可提高芯材渗漏量,加速脂质氧化;贮藏初期,光照组及空气接触组与对照组相差不大,可能与羧甲基壳聚糖能在一定程度上延缓体系的光致氧化及自氧化进程有关,但其保护作用有限,因此CM-PL应真空、避光、低温贮藏;复水稳定性结果显示CM-PL对氯化钠的耐受性高于葡萄糖,与二者共存贮藏14 d后CSO渗漏率分别为14.3%,21.1%。

The storage stability and water rehydtrate stabilty of carboxymethylchitosan- coated proliposomes （CM-PL）containing coix seed oil was studied. On one hand, coix seed oil （CSO）leakage rate and malondialdehyde（MDA） production during CM-PL stored under different conditions（ light, oxygen,temperature） was determined for storage stability evaluation.On the other hand ,the leakage rate of CSO after CM-PL rehydrated in sodium chloride solution or glucose solution was also measured for rehydtrated stabilty evaluation.The results indicated that the stability of CM- PL could be affected by light, higher temperature and air contact, and the influence was in the order of temperature 〉 air 〉 light.Higher temperature resulted in larger CSO leakage, which accelerated lipid oxidation further.The non significant difference among light treated or air contact groups with their control samples maybe related to the carboxymethylchitosan layer,which could delay the light-oxidation and auto -oxidation to some extent.However,the protection was limited, and CM-PL should be stored under vacuum, avoid light and low temperature condition.As to the other aspect, CM-PL exhibited better tolerance on sodium chloride than glucose,with the leakage ratio of 14.3% and 21.1% ,respectively after 14 days＇ storage.