摘要
在工程大肠杆菌生产羟基脂肪酸的基础上,为解决构建的以葡萄糖为底物合成羟基脂肪酸(HFAs)代谢途径中存在的细胞内还原力(NADPH)不平衡的问题,克隆了来源于巨大芽孢杆菌葡萄糖脱氢酶(GDH)基因,构建了胞内HFAs和NADPH联产的工程菌株;实验结果表明该重组大肠杆菌合成HFAs的产量得到显著提高,摇瓶条件下HFAs产量达到173.9 mg/L。具有较好的工业化开发前景。
A metabolic pathway to synthesize hydroxyfatty acids （HFAs）from glucose was constructed. But the reducibility（NADPH） imbalance hampers its application. In order to increase the yield of HFAs, the glucose dehydrogenase（GDH） from Bacillus megaterium was cloned and a NADPH and HFAs co-product strains was built to solve the reducibility imbalance in cell. Under shake-flask conditions, this recombinant strain produced 173.9 mg/L HFAs.The research laid the foundation in industrial applications for obtaining engineered Escherichia coil to produce HFAs with hi qh production.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第5期163-166,共4页
Science and Technology of Food Industry
基金
威海市科技发展计划(2014DXGJ40)
关键词
羟基脂肪酸
葡萄糖脱氢酶
基因工程
重组大肠杆菌
hydroxyfatty acids
glucose dehydrogenase
genetic engineering
recombinant Escherichia coli
