纤维素酶法提取荸荠皮中多糖的工艺研究

Study on the extraction technology of polysaccharide in water chestnut peel by cellulase

以低温烘干的荸荠皮为原料,利用纤维素酶法提取荸荠皮中的多糖,通过单因素实验和正交实验考察了p H、酶用量、液料比、酶解温度、酶解时间对多糖提取效果的影响。结果表明:当p H为5,酶用量为2.0%,液料比为30∶1 m L/g,酶解温度为60℃,酶解时间为2 h时,得率最高。在此条件下作了5次平行实验,平均得率可达32.33%,远大于相同条件下水提法的得率18.31%,而且实验重现性好。因此,用于荸荠皮中多糖提取的纤维素酶法是一种很有发展前途的提取方法。

With low-temperature baked water chestnut peel as raw material,the polysaccharide was extracted from the water chestnut peel by cellulase. The effect of the pH, the amount of cellulase, the liquid-solid ratio, the enzymolysis temperature, and the enzymolysis time, on the extraction of polysaccharide, were studied by the single factor experiments and the orthogonal tests.The results showed that when the pH was 5,the amount of cellulase was 2.0% ,the liquid-solid ratio was 30： ] mL/g,the enzymolysis temperature was60 ~C,the enzymolysis time was 2 h,the extraction rate reached the maximum.Under the optimal conditions,five parallel experiments were carried out and the extraction rate could reach 32.33% ,which was highly more than the extraction rate of 18.31% of water extraction method at the same conditions.The reproducibility of the experiments was good.So the cellulase method used for extraction of polysaccharide in water chestnut peel,was one kind of promising extracting method.