期刊文献+

石斛内生菌SH-1 PQQ基因的克隆与生物信息学分析

Cloning and Bioinformatic Analysis of PQQ Gene from Klebsiella variicola SH-1
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摘要 从石斛内生菌变栖克雷伯菌(Klebsiella variicola)SH-1中PCR扩增得到吡咯喹啉醌(PQQ)基因,其大小为5 444 bp,包含了pqqABCDEF 6个基因,并对PQQ基因进行了生物信息学分析,为下一步构建高效基因工程菌,研究其生物合成途径奠定了基础。 Pyrroloquinoline quinon(PQQ) genes was amplifed from an endophytic bacteria Klebsiella variicola SH-1,which was isolated from Dendrobium candidum. A 5 444 bp DNA fragment contained six genes. The bioinformatics analyses of PQQ genes laid the foundations for the further steps to structure efficiently genetic engineering strians and study the biosynthetic pathway of PQQ.
出处 《湖北农业科学》 2016年第2期490-492,514,共4页 Hubei Agricultural Sciences
基金 国家自然科学基金地区基金项目(81360611) 农业科技成果转化资金项目(2013GB2F300441)
关键词 PQQ基因 变栖克雷伯菌 生物信息学分析 PQQ gene Klebsiella variicola bioinformatic analysis
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