丹磺酰氯对异丙酚荧光衍生化的工艺研究

Study on preparation process of propofol fluorescence derivatization by dansyl chloride

目的以丹磺酰氯(DNS-Cl)作为荧光衍生化试剂对异丙酚进行荧光衍生化方法的研究与探讨。方法DNS-Cl对异丙酚荧光衍生化后,衍生产物用荧光分光光度法检测,通过单因素变量法考察衍生化条件。结果当DNS-Cl与异丙酚的摩尔比为8∶1,在浓度为0.1mol/L氢氧化钠碱性条件下60℃水浴避光反应5min后,衍生产物用环己烷萃取1min效果最佳。结论该荧光衍生化条件温和、反应速度快,DNS-Cl可以应用于异丙酚的衍生化。

Objiective To explore preparation process of fluorescence derivatization for propofol by using dansyl chloride as derivatization reagent.Methods Utilize the reaction of propofol and the DNS-Cl to obtain a fluorescent derivative product which was detected by fluorospectrophotometry.And investigate derivatization conditions by single-factor variable method.Results The results of derivatization indicated that the derivatives could be obtained by the labeling reaction of DNS-Cl with propofol（DNS-Cl：propofol=8：1in mole ratio）in the presence of sodium hydroxide（0.1mol/L）at 60℃for 5min in the dark,then using cyclohexane to extract for 1min.Conclusion DNS-Cl could be applied to derivatize for propofol,the reaction condition was mild and the reaction velosity is fast in the fluorescence derivatization.