摘要
以毛竹基因组数据库中的107个编码b ZIP转录因子的基因为材料,对其进行系统发育分析,筛选得到了A、D和S亚族的b ZIP家族成员,并对其基因结构、适应性进化、与水稻基因对之间的进化分歧时间及蛋白质同源建模等进行生物信息学分析。结果表明,毛竹A、D和S亚族共有47个b ZIP家族成员,其中A亚族18个,D亚族16个,S亚族13个。基因结构分析表明,3个亚族中内含子数量为0-16个,而且D亚族基因的内含子—外显子组成比A亚族和S亚族更为复杂。适应性进化分析表明,3个亚族总体上处于净化选择压力之下;A、D和S亚族的平均进化分歧时间分别为279.0,265.6和202.9 Mya;47个毛竹b ZIP蛋白质主要以α-螺旋为主,且D亚族的三级结构比A和S亚族复杂。
Genes of 107 PhyUostachys edulis bZIP transcription factors were collected as materials from available public databases. Subsequently, the A, D and S subgroup members were screened through the phylogenetic analysis, and their physico-chemical property, gene structure, adaptive evolution, and the evolutionary divergence time were further analyzed by the bioinlormatics method. The results showed that the A, D and S subgroups of P. edulis bZIP transcription factors contained 47 members of them, 18 bZIPs belonged to the A subgroup, 16 and 13 belonging to D and S subgroup, respectively. Gene structure analysis showed that, among these bZIP genes, the number of introns ranged from 0 to 16, and the composition of intron-exon in D subgroup genes was mm'e complex than that of A and S subgroups. Meanwhile, according to adaptive evolution analysis, it was discovered that these three P. edulis bZIP subgroups undergone purifying selection pressure, and the mean evolutionary divergence time in the A, D amt S subgroup was 279.0, 265.6 and 202.9 Mya, respectively. Finally, via protein tertiary structure analysis it was discovered that, 47 bZIP proteins were mainly made up of α-helix and the members of D group were more complex than that of S and A group.
出处
《森林与环境学报》
CSCD
北大核心
2016年第1期54-61,共8页
Journal of Forest and Environment
基金
国家自然科学基金青年基金项目"超量表达NaSUSs基因对梁山慈竹纤维素生物合成调控效应的研究"(31400257)
四川省应用基础研究基金项目"4CL
CCoAOMT
C3H基因调控工业用竹的研究"(2013JY0182)
西南科技大学研究生创新基金项目"慈竹bZIP转录因子的克隆及其遗传转化研究"(15ycx084)
关键词
毛竹
bZIP转录因子
生物信息学分析
Phyllostachys edulis
bZIP transcription factor
bioinformatics analysis