摘要
以秋茄(Kandelia obovata)幼苗为材料,研究了喷洒0.1 mmol·L^(-1)烯效唑对植株叶片光合作用、叶绿素、荧光特性、抗氧化系统及细胞膜透性的影响。结果表明:烯效唑能显著提高低温胁迫下秋茄幼苗叶片净光合速率(P_n)、气孔导度(G_s)、叶绿素含量和叶绿素a/叶绿素b(Chl a/Chl b)比值,降低胞间CO_2浓度(C_i)和气孔限制值(L_s);同时烯效唑也能显著提高低温胁迫下秋茄幼苗叶片PSⅡ最大光化学效率(F_v/F_m)、实际光化学效率(Φ_(PSⅡ))、光化学猝灭系数(q_P),增加了超氧化物歧化酶(SOD)、过氧化物酶(POD)与抗坏血酸过氧化物酶(APX)活性,反而降低了非光化学猝灭系数(NPQ)、细胞膜透性和丙二醛(MDA)积累,说明烯效唑处理能改善低温胁迫下秋茄植株抗氧化系统运行,增强其植株光合电子传递效率,从而有效促进植株的光合作用,减少低温胁迫对秋茄叶片细胞的伤害。
The effect of uniconazole on leaf photosynthetic characteristics,chlorophyll fluorescence and antioxidant system in Kandelia obovata seedling under cold stress was investigated.Kandelia obovata seedlings were pretreated with 0.1 mmol·L^-1 uniconazole for 7 d,and then exposed to cold stress at day/night of(5/-2)℃ for48 h.Results show that the net photosynthetic rate(Pn),stomatal conductance(Gs),chlorophyll content and Chl a/Chl b ratio significantly increased,and intercellular CO2 concentration(Ci) and stomatatal limitation(Ls) were reduced in K obovata leaves by the use of uniconazole.The photochemical efficiency of photosystem Ⅱ(Fv/Fm),actual photochemical efficiency(ΦPSⅡ) and photochemical quenching cofficient(qP) could be enhanced,but non-photochemical quenching coefficient(NPQ) decreased,in K obovata leaves with uniconazole treatment.In addition,uniconazole alleviated the oxidative damage in leaves of K obovata by reducing cell membrane permeability and lipid peroxidation,and by increasing the activities of superoxide dismutase(SOD),ascorbate peroxidase(APX) and peroxidase(POD).Sum up,it indicates that uniconazole alleviates freezing-induced cell damages through regulating the photosynthesis and antioxidant system of K.obovata.
出处
《植物生理学报》
CAS
CSCD
北大核心
2016年第1期109-116,共8页
Plant Physiology Journal
基金
海洋公益性行业科研专项经费项目(20150528)
浙江省重大科研专项(2012C12017-3)
浙江省自然科学基金(LQ13C030002)
浙江省科技计划项目(2013C25096和2014F50003)
浙江省远洋渔业(2015)“基于生态系统水平的中以(中国-以色列)海水养殖技术合作与交流”
关键词
红树林
秋茄
烯效唑
低温胁迫
光合作用
抗氧化系统
mangrove
Kandelia obovata
uniconazole
low temperature stress
photosynthesis
antioxidant system