电针对非酒精性脂肪性肝病大鼠蛋白质二硫化物异构酶A3的影响

Effect of Electroacupunctrue on ERp57 in NAFLD Rats

目的研究电针对非酒精性脂肪肝(NAFLD)大鼠内质网应激标志物蛋白质二硫化物异构酶A3(ERp57)的影响,探讨电针治疗NAFLD的作用机制。方法将60只雄性SD大鼠随机分为普食组(n=15)和高脂饮食组(n=45),高脂饮食组饲以高脂饲料建立NAFLD动物模型,5周后两组各取2只大鼠对比验证造模。造模成功后,普食组随机抽取10只作为正常对照组,高脂饮食组再随机分为4个组(每组各10只),其中饮食1组和电针1组继续饲以高脂饮食,饮食2组和电针2组饲以普通饮食。电针1组和电针2组毫针针刺"足三里""三阴交""太冲"三穴,其中"足三里""三阴交"接通电针刺激,每日1次,单侧交替取穴,每次治疗20min,连续4周。每周称取各组大鼠体质量,治疗结束后观察各组大鼠血脂及肝功的变化,RT-PCR和Western blot检测ERp57的基因和蛋白表达,并检测下游分子固醇调节元件结合蛋白-1c(SREBP-1c)的基因表达。结果 4周后,饮食1组大鼠体质量增长低于饮食2组、电针1组和正常对照组(P<0.05);电针2组大鼠体质量增长高于电针1组(P<0.05),与饮食2组差异不明显(P>0.05)。饮食1组血清血脂、肝功指标以及肝脏ERp57和SREBP-1c基因和ERp57蛋白的表达高于正常对照组,电针1组和饮食2组(P<0.05);电针2组分别与饮食2组和电针1组比较,上述指标降低更为明显(P<0.05)。结论电针能有效抑制ERp57的表达,改善NAFLD大鼠肝脏内质网应激,从而降低SREBP-1c的表达,改善脂质代谢,这可能是电针治疗NAFLD的机制之一。

Objective To investigate the effect of electroacupunctrue on ERp57 in non-alcoholic fatty liver disease（NAFLD）rats,and study the therapeutic mechanism of electroacupunctrue in patients with NAFLD.Methods Sixty male SD rats were randomly divided into common diet group（n=15）and high-fat diet group（n=45）.5weeks later,two rats from the two groups were executed and confirmed that the model was successful.Then10 rats in common diet group were chosen as control group（Control）,and 40 rats in high-fat diet group were randomly chosen and divided into diet group 1（D1）,diet group 2（D2）,electroacupuncture group 1（EA1）and electroacupuncture group 2（EA2）（n=10each）.D1 and EA1were fed by high fat diet;D2and EA2 were fed with common diet.In EA1 and EA2,filiform needle acupuncture was applied to ST36,SP6 and Liv3 and electroacupunctrue was applied to one-side of ST36,SP6 for 20min once daily for 4weeks.The rats in each group were weighed per-week.After the treatment the changes of blood lipid and liver functions of these rats were observed.ERp57 gene expression and protein expression were detected by RT-PCR and Western blot,and expression of ERp57 downstream SREBP-1cwas detected.Results The body mass of D1 increased slowly and were lower than D2 and EA1（P0.05）;the body weights of EA2 increased rapidly and were higher than EA1（P0.05）,but without significant difference with D2（P0.05）.The contents of blood lipid,liver functions and the expression of ERp57 and SREBP-1cwere significantly higher than those in Control,D2 and EA1（P0.05）.While compared to D2 and EA1respectively,the index mentioned above in EA2 decreased more significantly（P0.05）.Conclusion Electroacupunctrue can decrease expression of ERp57 to improve endoplasmic reticulum stress（ERS）of rats with NAFLD and then decrease expression of SREBP-1cto regulate rat lipid,which could be one of mechanism to cure NAFLD.