摘要
[Objective] This study aimed to extract magnolol from wild Magnolia offici- nalia leaves by ultrasonic-assisted extraction. [Method] Magnolol was qualitatively id- entified by coloration method and thin layer chromatography, and magnolol content in Magnolia officinalia leaves was measured by high performance liquid chromatog- raphy （HPLC）. The HPLC was conducted with C18 as the stationary phase while different mobile phases were selected. The measurement wavelength, flow velocity and sample size adopted in the HPLC were 294 nm, 1 ml/min and 20 μl, respec- tively. [Result] Magnolol content in Magnolia officinalia leaves was 0.75%. When methyl alcohol and water with a volume ratio of 78：22 was used as the mobile phase, the retention time of magnolol was 4.528 min and the separation effect was good. In addition, it was easy to operate with good reproducibility and sensitivity. [Conclusion] This method is suitable for the measurement of magnolol content in Maonolia officinalia leaves.
[目的]用超声辅助法提取野生厚朴树叶中的厚朴酚。[方法]用显色法和薄层色谱法对厚朴酚进行定性鉴定,高效液相色谱法测定厚朴树叶中厚朴酚的含量,以C_18 为固定相,选择不同的流动相,检测波长294 nm,流速为1ml/min,进样量20μl。[结果]厚朴树叶中含有厚朴酚,厚朴酚的含量为0.75%,以甲醇︰水的体积比78︰22为流动相,测得厚朴酚的保留时间为4.528 min,分离效果好,简单,灵敏,重现性好,结果满意。[结论]该方法适合于厚朴树叶中厚朴酚含量的测定。
基金
Industrialization Cultivation Project of Shaanxi Province Education Department(2012JC09)
Scientific and Technological Project of Social Development of Shaanxi Science and Technology Agency(2015SF270)~~
