FUNDC1基因沉默对卵巢癌SKOV3细胞增殖和侵袭的影响

Effects of silencing FUNDC1 gene on proliferation and invasion of ovarian cancer cell SKOV3

目的探讨FUNDC1蛋白在卵巢癌组织中的表达及其对卵巢癌细胞增殖和侵袭的影响。方法通过免疫组织化学(S-P法)检测FUNDC1蛋白在卵巢癌组织中的表达;流式细胞仪检测卵巢癌SKOV3细胞沉默FUNDC1基因后细胞周期的变化;Ed U检测卵巢癌SKOV3细胞沉默FUNDC1基因后细胞增殖能力变化;Transwell检测卵巢癌SKOV3细胞侵袭能力;Western blotting检测p21和p53蛋白的表达。结果 FUNDC1蛋白在卵巢癌组织细胞质中表达;在卵巢癌组织中FUNDC1蛋白呈阳性表达,在正常卵巢组织中FUNDC1蛋白呈弱阳性或不表达。与正常卵巢组织相比,FUNDC1蛋白在卵巢癌组织中的表达明显增高(P=0.021);FUNDC1蛋白在晚期卵巢癌(Ⅲ/Ⅳ期)组织中的表达明显高于早期卵巢癌(Ⅰ/Ⅱ期)组织,差异有统计学意义(P=0.012)。沉默卵巢癌SKOV3细胞FUNDC1基因后,卵巢癌细胞增殖能力(P=0.032)和侵袭能力(P=0.035)降低,S期细胞明显减少(P=0.015),p21和p53蛋白表达上调。结论 FUNDC1蛋白在卵巢癌组织中的表达上调,FUNDC1调控卵巢癌SKOV3细胞增殖可能和p53/p21途径有关。

Objective To explore the expression of FUNDC1 protein in tissues of ovarian cancer and its effects on proliferation and invasion of ovarian cancer cells.Methods The expression of FUNDC1 protein in tissues of ovarian cancer was detected by immunohistochemical（S-P method）.Changes of the cell cycle of ovarian cancer cell SKOV3 were detected by flow cytometry after the FUNDC1 gene was silenced.The abilities of proliferation and invasion of ovarian cancer cell SKOV3 were detected by EdU assay and Transwell assay,respectively.Western blotting was used to detect the expressions of p21 and p53 proteins.Results FUNDC1 expressed in the cytoplasm of ovarian cancer tissues.FUNDC1 positively expressed in tissues of ovarian cancer,while showed weakly positive or negative expression in normal ovarian tissues.Compared with normal ovarian tissues,the expression of FUNDC1 in ovarian cancer tissues was significantly higher（P = 0.021）.The expression of FUNDC1 in advanced ovarian cancer tissues（stage Ⅲ/ Ⅳ） was significantly higher that in early stage ovarian cancer tissues（stage Ⅰ/ Ⅱ） and the difference was statistically significant（P = 0.012）.After the FUNDC1 gene of ovarian cancer cell SKOV3 was silenced,the abilities of proliferation（P = 0.032） and invasion（P = 0.035） of ovarian cancer cells decreased.Cells at S phase decreased significantly（P = 0.015） and expressions of p21 and p53 proteins up-regulated.Conclusion The expression of FUNDC1 protein in ovarian cancer tissues is up-regulated.The regulation of proliferation of ovarian cancer cell SKO V3 by FUNDC 1 may be relevant to p21/p53 pathway.