耐有机溶剂α-葡萄糖苷酶的补料分批发酵研究

Enhanced Production of Solvent-Stable Alpha Glycosidase from Bacillus licheniformis JXC-1 by an Optimal Feeding Strategy

作者研究了Bacillus licheniformis JXC-1在3 L发酵罐上产耐有机溶剂α-葡萄糖苷酶的关键因素,结果表明：p H恒定7.0,初始糖质量浓度25 g/L,搅拌转速600 r/min为最适产酶条件,酶活由104.6 U/L提高到了444.7 U/L。通过4种补料策略对其进行补料分批发酵研究,得出：4-5 h内补料速度为2.25 m L/h,5-6 h内补料速度为6.75 m L/h,6-7 h内补料速度为9 m L/h,7-8 h内补料速度为15 m L/h补加麦芽糖与蛋白胨效果最好,酶活达到872.5 U/L。

In previous work,we screened a strain（Bacillus licheniformis JXC-1） producing solvent-stable（10% N,N-Dimethylformamide,short for DMF） α-glucosidase from several different soil samples. In this work,we attempted to determine the key factors for the production ofα-glucosidase and optimize the feeding strategy in a 3-L fermenter. Results showed that the enzyme activity reached 444.7 U/L with the optimal condition（p H 7.0,the initial maltose concentration 25g/L,and agitation speed 600 rpm）. The strategy of feeding maltose and tryptone at a rate of 2.25 m L/h（4-5 h）,6.75 m L/h（5-6 h）,9 m L/h（6-7 h） and 15 m L/h（7-8 h） significantly increased the production of α-glycosidase from 444.7 U/L to 872.5 U/L.