产油微藻的分离筛选与鉴定

Isolation and Characterization of Oil-Producing Microalgae

微藻所含的油脂是制备生物柴油的理想原料。为了筛选具有产油能力的微藻,作者从自然界水体中分离出8株微藻,对他们的生长特性和产油能力进行评价,旨在筛选出生长速度快、生物量高、油脂含量高的优良藻种。实验结果得到一种油脂产率较高的CC-B3藻株,它的总脂质量分数为36.30%,生物量达到2.43 g/L,油脂产率为62.9 mg/(L·d)。符合优良藻种的条件,是一株高效产油微藻。通过18s r DNA鉴定,该藻株为斜生栅藻(Scenedesmus obliquus),构建了该藻株的进化树。

The oil contained in the microalgae was considered as an ideal raw material for biodiesel.In order to screen microalgae that have the potential to produce oil,eight strains of microalgae were isolated from natural water and evaluated for their growth,biomass and fat content. A highly efficient oil-producing microalga CC-B3 was found with the total lipid content 36.30%,biomass 2.43 g/L and oil yield 62.9 mg/（L·d）. Base on the 18 s r RNA,the strain of algae was identified to be Scenedesmus obliquus and its phylogenetic tree was thus constructed.