有机溶剂处理提高固定化脂肪酶活性及稳定性

Treatment of Organic Solvents to Improve Activity and Stability of Immobilized Lipases

为提高固定化脂肪酶催化转酯化反应的活性和稳定性,采用活性炭、硅胶G和DM-130树脂吸附固定脂肪酶LVK-F100,并用甲醇、丙酮、丙醇、异丙醇、乙酸乙酯、正己烷以及环己烷处理湿的固定化脂肪酶。结果表明,甲醇、正己烷和环己烷处理对固定化脂肪酶有钝化作用,而丙酮、丙醇、异丙醇和乙酸乙酯处理能显著提高固定化脂肪酶的活性。用乙酸乙酯和异丙醇处理吸附固定的脂肪酶1 h,可使固定化脂肪酶活力分别提高1.70-2.25倍、半衰期延长3.2-8.4 h、催化大豆油转酯化反应延长使用周期2-5批。异丙醇和乙酸乙酯处理还可提高固定化脂肪酶的热稳定性。

The lipases immobilized on active carbon,silica gel G or DM-130 macro porous resin by adsorption were steeped in various organic solvents to improve the activity and stability for catalyzing transesterification. It was found that the activities of immobilized lipases could be increased with acetone,1-propanol,2-propanol and ethyl acetate,but could be deactivated with methanol,n-hexane and cyclohexane. Compared with the immobilized lipases dried directly after adsorption,the immobilized lipases treated in 2-propanol or ethyl acetate for 1 h displayed 1.70 to2.25 times enzyme activities,their half-lives were prolonged 3.2 to 8.4 h and the operation lives were increased 2 to 5 batches for soybean oil transesterification. The thermal stability of the immobilized lipases could also be enhanced by the treatment with 2-propanol or ethyl acetate.