介孔硅酸钙／硫酸钙骨水泥修复兔创伤性骨缺损

Study on mesoporous calcium silicate/calcium sulfate bone cement for repair of traumatic bone defect

目的观察新型介孔硅酸钙（m-CS）／硫酸钙骨水泥（CSC）复合骨水泥（m-CSC）修复骨缺损的效果。方法以CSC为对照组，以15m-CSC为实验组1、30rn—CSC为实验组2，观察骨水泥的凝结时间和抗压强度。将骨水泥样品浸泡于Tris-HCl溶液中，观察其体外降解性能、细胞形态及细胞在样品表面的增殖及分化情况。将骨水泥植入9只新西兰大白兔股骨创伤性骨缺损，于术后4，8，12周取样，通过组织切片分析骨水泥的降解及成骨性能。结果实验组1和实验组2的固化时问分别为7．8rain和10．5min，明显长于对照组的3．7min（P〈0．01），且对实验组1和2的抗压强度影响很小。实验组1和实验组2在浸泡液的失重率分别为61．8wt％和50．3wt％，明显低于对照组的70．4wt％（P〈0．01）；其中实验组2浸泡液的pH值下降缓慢（pH值从7．40下降到7．26），而对照组下降较快（pH值从7．40下降至6．86）（P〈0．01）。与对照组比较，实验组1和实验组2细胞增殖和分化增强，治疗兔股骨创伤性骨缺损12周，实验组2的新生骨组织面积占55．2％，成骨量显著高于对照组的25．6％（P〈0．01）。结论m-CSC具有优良的生物相容性、降解性和成骨性能，能促进新骨再生，有效修复创伤性骨缺损。

Objective To analyze the effect of mesporous calcium silicate （m-CS）/calcium sultate cement （CSC） , m-CSC for short, in bone defect repair. Methods Setting time and compressive strength of the m-CSC （ 15 m - CSC as group Ⅰand 30 m - CSC as group Ⅱ ） were tested. CSC was used as the control. Cement samples were immersed in Tris-HCl solution, andin vitro degradation of the m-CSC was measured. Cell morphology and cell proliferation as well as differentiation on the samples were assessed. The cements were implanted into the traumatic femoral defects in rabbits, and the in vivo degradability and osteogenesis of the cements were investigated by histological evaluation after implantation for 4, 8 and 12 weeks. Results Addition of m-CS into CSC prolonged the setting time （7.8 min in group I and 10.5 min in group Ⅱ ） , obviously longer than 3.7 rain in control group and did not have obvious effect on compressive strength of the cements. Weight loss of m-CSC solution was obviously lower （61.8 wt% in group I and 50.3 wt% in group Ⅱ ） , compared to 70.4 wt% in control group, pH value in group Ⅱ decreased from 7.40 to 7.26, while decreased from 7.40 to 6.86 in control group, m-CSC could promote cell proliferation and differentiation compared to CSC. At postoperative 12 weeks, histolog- ical sections showed massive new bony tissue （55. 2% ） in group Ⅱ , obviously higher than 25.6% in control group. Conclusion m-CSC exhibits good biocompatibility, degradability and osteogenesis,and can promote bone regeneration in bone defect repair.