同轴静电纺丝法制备具有核-壳纤维结构的多功能敷料

Preparation of Multi-functional Wound Dressing with Core-shell Nanofibers via Co-axial Electrospinning

利用同轴静电纺丝制备了具有核壳结构纳米纤维的未交联敷料,其中纤维内核为载有抗菌药物莫匹罗星的聚己内酯(PCL),外壳则由载有麻醉剂利多卡因的胶原构成;通过京尼平将胶原外壳交联后得到交联敷料.用扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察了未交联敷料的表面形貌和纤维的核壳结构.体外药物释放实验结果表明,在2种敷料中,2种药物在1 h内均出现了突释现象,而在随后的60 h中,2种药物均能从敷料中缓慢释放出来,说明2种敷料均具有较好的持续止痛与抗菌性能.二辛可宁酸(Bicinchonininc acid,BCA)蛋白测试结果表明,未交联敷料外壳上的胶原蛋白能够持续地释放出来.体外细胞培养结果表明,与交联敷料相比,未交联敷料能够更好地促进成纤维细胞L929的黏附和生长,具有更好的促进伤口愈合作用.体外抗菌实验结果显示,负载了莫匹罗星的2种敷料的抗菌性能均明显高于对照组,具有良好的抗菌性能.

The uncrosslinked wound dressing with core-shell nanofibers was firstly fabricated via co-axial electrospinning. The nanofiber was composed of the polycaprolactone（ PCL） core loaded with anti-bacterium drug mupirocin and collagen shell with anesthesia lidocains. The surface morphology of uncrosslinked dressing and its core-shell structured fibers were characterized by scanning electronic microscopy and transmission electronic microscopy,respectively. The crosslinked wound dressing can be further prepared by the crosslinking of collagen shell,and the successful crosslinking can be proved by the enhanced tensile strength and swelling ratio. In vitro drug release tests implied two dressings both possessed the burst release stage and sustainable release stage for two loaded drugs,which confirmed the sustainable acesodyne and anti-bacteria effects for both dressings. The bicinchonininc acid（ BCA） test results showed that the collagen in uncrosslinked dressing could also be sustainably released,while in vitro cell attachment test depicted that uncrosslinked dressing could promote the cell attachment and growth better than crosslinked dressing. Furthermore,the in vitro anti-bacteria test showed that both dressings loaded with mupirocin had significantly better anti-bacteria ability than those without mupirocin indicating their excellent anti-bacteria ability. Two as-prepared multifunctional wound dressings possessed acesodyne and anti-bacteria effects and were able to promote the healing process so that they can reduce the pains of patients,reduce the number of dressing change,and enhance treatment effect.