基于对羟基苯硼酸为前驱体的金纳米粒子修饰电极电化学检测过氧化氢

Electrochemical Detection of Hydrogen Peroxide at Au NPs Modified Electrode Using p-Hydroxyphenylboronic Acid as a Precursor

以对羟基苯硼酸为前驱体,利用过氧化氢(H_2O_2)可以定量氧化对羟基苯硼酸产生对羟基苯酚的原理,以反应产物对羟基苯酚为电化学信号物质,结合金纳米粒子修饰玻碳电极(AuNPs/GCE),发展了一种间接检测H_2O_2的电化学方法.由于AuNPs/GCE具有有效电子传递性能和比表面积大等优点,对硼酸氧化产物具有较高的催化活性,因此在含1.0 mmol·L^(-1)对羟基苯硼酸的0.1 mol·L^(-1)pH 7.5 PBS中,AuNPs/GCE可以检测到1.0~1.0×10~3μmol·L^(-1)的H_2O_2,检测限为0.5μmol·L^(-1).同时,该方法具有良好的选择性和重现性,且操作简单、速度快、价格低廉,非常适用于实际样品中H_2O_2含量的测定.

We developed a new method for electrochemical detection of hydrogen peroxide （H202） based on boronate oxidation of p-hydroxyphenylboronic acid. This method using p-aminophenol which is produced from the reaction of H2O2 and p-aminophenylboronic acid as a well electrochemical probe, combined with a gold nanoparticles （AuNPs） modified electrode for an indirect detection of H2O2. Because of the large surface area and enhanced electrocatalytic behavior by the AuNPs modified electrode, the detection sensitivity was improved. The method could detect H2O2 in the concentration range of 1.0 - 100 μmol. L-1 and 0. 1 - 1.0 mmol.L-1 in 0.1 mol. L-1 pH 7.5 PBS containing 1.0 mmol. L-1 p-hydroxyphenylboronic acid. The low detection limit was 0.5 μmol. L-1. The proposed method had good selectivity and stability. Moreover, the method was quick, simple and cheap, which has potential application in real samples analysis.