牛至精油β环糊精微胶囊的制备及其抑菌效果研究

Preparation of Oregano Essential Oil / Beta-cyclodextrin Microcapsule and Its Antimicrobial Activity

目的制备牛至精油β环糊精(β-CD)微胶囊,并研究其抑菌效果。方法首先,选用β-CD包埋牛至精油,通过扫描电镜观察该包埋物的外部形貌特征。其次,通过薄层色谱、红外光谱等2种方法考察是否已成功制备牛至精油β环糊精的微胶囊。然后,通过紫外分光光度计测定该包埋物的吸光度,按照一定的方法计算其包埋率。最后,选用大肠杆菌、米曲霉菌作为供试菌株,研究该包埋物的抑菌效果。结果牛至精油β环糊精微胶囊得到成功制备,其包埋率为73%,并且该包埋物具有较好的抑菌效果。结论实验达到了预期目的,即在保留牛至精油抑菌性能的同时,也使得液体的牛至精油固体粉末化。

The objective of the test was to prepare the microcapsule of beta-cyclodextrin / oregano essential oil and study its antimicrobial activity. First, Beta-cyclodextrin was selected as the embedding material to get the oregano oil microcapsule. Second, SEM was used to observe the external appearance of the inclusion complex. Next, the two methods of TLC and IR were used to check whether the complex was successfully prepared or not. Then the embedding rate was determined with the help of UV spectrophotometer and some certain processions. Last, significant antimicrobial activities of the microcapsule were tested against Escherichia coli and Aspergillus oryzae. The microcapsule of beta-cyclodextrin /oregano essential oil was successfully prepared with an embedding rate of 73%, and it had a good antimicrobial effect. The experiment has achieved its expecting purpose, i.e., preparing the liquid of oregano essential oil into be solid power and meanwhile keeping the oil′s antimicrobial activity.