摘要
本研究的目的是构建广西巴马小型猪SAP真核表达载体并在胚胎水平对其进行验证。利用RT-PCR技术扩增并克隆SAP基因,再用T4连接酶将其连入P-Dsred-N1载体,构建P-Dsred-N1-SAP载体,并通过胞质注射生产转SAP的猪胚胎。结果表明,本研究成功构建了P-Dsred-N1-SAP重组载体,经胞质注射后在胚胎发育早期和囊胚期均有红色荧光表达。本研究为下一步研究SAP基因的功能和生产转SAP基因猪奠定基础。
The objective of this study was to construct the eukaryotic expression vector of SAP from Guangxi Bama mini-pig and to be validated in the embryos level. The coding sequence of SAP gene was amplified from pancreas of Guangxi Bama mini-pig by RT-PCR and the SAP fragments were inserted into P-Dsred-N1 vector by T4 ligase to construct the P-Dsred-N1-SA P expression vector. Furthermore, SAP transgcnic cloned porcine embryos were produced by cytoplasmic injection. The results showed that P-Dsred-N1-SA P vector was constructed succ- essfully and the red fluorescence was observed in the development of early embryos and blastocyst stage embryos by cytoplasm injection. In conclusion, this study laid a foundation for researching the function of SAP and prod- ucing the SAP pig in future.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2016年第1期53-57,共5页
Genomics and Applied Biology
基金
广西科技基础条件平台建设项目(09-090-09)
国家现代农业产业技术体系广西生猪创新团队项目(nycytxgxcxtd-03-15)共同资助
