4个贵州地方猪品种MKLN1基因中SNP位点的多态性研究

Polymorphism of SNP Locus at MKLN1 Gene in Four Native Pig Breeds of Guizhou Province

本研究在香猪和柯乐猪单核苷酸多态性(single nucleotide polymorphism,SNP)芯片筛查的基础上,采用AS-PCR技术,以香猪、柯乐猪、糯谷猪、黔北黑猪4个贵州地方猪品种为研究对象,研究muskelin 1基因第18外显子中rs81213998(DIAS0003365)位点的多态性与体尺指标之间的关系。结果显示,MKLN1第18外显子中rs81213998位点基因型在4个猪群中有多态性。香猪的C等位基因频率明显高于其它3个猪品种的相应值(p<0.01),T等位基因的频率较低(p<0.01),黔北黑猪、糯谷猪和柯乐猪的C和T之间的差异不明显(p>0.05)。相关性分析显示,rs81213998位点与猪的管围、体长、体高、体宽呈弱的正相关。推测MKLN1基因的rs81213998位点可能与贵州地方猪品种的体尺指标之间有一定的关系。

Based on t he data of porcine 60K SNP chip for Xiang and Kele pigs, the polymorphisms of site rs81213998 （DIAS0003365） located at exonl8 ofMKLN1 gene were detected by AS-PCR method in this study. Four native pig breeds of Guizhou were chosen as experiment samples which included Xiang pig, Kele, Nuogu, Qian-bei black. The relationship of SNP genotypes with the body measurement size were analyzed among four native pig breeds of Guizhou. The results showed that the genotypes of rs81213998 were much diverse in four pig breeds. The frequencies were significantly higher in allele C and lower in allele T of Xiang pig than that of Kele, Nuogu and Qianbei black pigs. The difference was not distinct between the other breeds besides Xiang pig. Using correlation analysis, it revealed that the genotypes of site rs81213998 were weak related to the pipe circumference, body length, body height and body width of pig populations. It suggested that the locus rs81213998 at MKLN1 gene might be associated with the indexes of body measurement in native pig breeds of Guizhou province.