中华鳖(Trionyx sinensis)微卫星标记与生长性状的相关分析

Correlation Analysis of the Microsatellite DNA Markers and Growth Traits of Chinese Soft Shell Turtle(Trionyx sinensis)

利用筛选到的25个微卫星标记,以中华鳖极大、极小两个群体共120个个体为实验材料,分析这些微卫星标记与体重、背甲长、背甲宽、体高、腹甲长及腹甲宽之间的相关性。结果表明:有3个位点与生长性状的相关性达到显著或极显著水平(p<0.05或p<0.01),其中位点LTF1与体重、背甲长、背甲宽、腹甲长、腹甲宽、体高均极显著相关;位点LTR87与体重、背甲长、和腹甲宽显著相关;位点LT8与体重、体高、腹甲长、腹甲宽显著相关。将同一性状不同基因型之间进行多重比较,结果显示位点LTF1中的BE型,位点LTR87中的BB型,以及位点LT8中的CF型、BC型、AC型、CC型具有明显的生长优势,可作为辅助育种的参考标记。

With a total of 120 Chinese soft shell turtle of maximum, minimum two groups individual as exp- erimental material, we analyzed the correlation between 25 selected microsatellite DNA markers and body weight, carapace length, carapace width, body height, shell length and shell width of Chinese soft shell turtle. The results indicated that 3 microsatellite loci showed significant or highly significant influences on these growth traits （p 〈 0.05 or p 〈0.01）. LTF 1 is significantly correlated with body weight, carapace length, carapace width, body height, shell length and shell width; and LTR87 is significantly correlated with body weight, carapace length and shell width, while LT8 is significantly correlated with body weight, body height, shell length and shell width. Multiple comparison analysis of genotypes related to growth traits showed that BE genotypes of LTF1, BB genotypes of LTR87, and CF, BC, AC and CC genotypes of LT8 played positive roles in growth traits respectively, which could give effective basis to the study of Chinese soft shell turtle marker-assisted breeding.