利湿活血方对高尿酸血症模型大鼠血管内皮功能的影响

Lishi Huoxue formula to protect the vascular endothelial cells against hyperuricemia in rats

目的研究利湿活血方及其拆方对高尿酸血症大鼠血清尿酸(UA)、一氧化氮(NO)、内皮素(ET-1)、血栓烷素B_2(TXB_2)及6酮前列腺素(6-keto-PGF_(1α))含量以及对高尿酸血症大鼠主动脉形态的影响,进而探讨利湿活血法对高尿酸血症模型大鼠血管内皮功能保护作用及其机制。方法将雄性SD大鼠随机分为空白组、模型组、苯溴马隆组,四妙丸组及利湿活血方组,拆方I组,拆方II组共7组。除空白组外,其余各组均以腺嘌呤和酵母膏灌胃,制备高尿酸血症模型,验模成功后,各给药组灌胃给予相应的药物,空白组和模型组给予等体积蒸馏水,连续14 d。末次给药后,采用紫外分光光度法检测大鼠血清UA及NO的含量,放射免疫分析法检测大鼠血清ET-1、TXB_2及6-ketoPGF_(1α)的含量,主动脉胸腹段HE及Masson染色。结果与空白组相比,模型组大鼠血清UA、ET-1、TXB_2含量显著升高(P<0.05),NO、6-keto-PGF_(1α)含量显著降低(P<0.05)。与模型组相比,利湿活血方组大鼠血清UA、ET-1、TXB_2含量降低(P<0.05),拆方Ⅰ组血清UA及ET-1含量降低(P<0.05);与模型组相比,利湿活血方组及其拆方Ⅰ、Ⅱ组大鼠血清NO、6-keto-PGF_(1α)含量显著升高(P<0.05)。与利湿活血方组相比,拆方Ⅰ、Ⅱ组在降低TXB_2含量方面效果不明显,拆方Ⅱ组在降低ET-1含量方面效果不明显。利湿活血方组及其拆方Ⅰ、Ⅱ组对高尿酸血症大鼠主动脉形态分别有不同程度的保护作用,以全方的效果最为明显。结论利湿活血方在降低高尿酸血症大鼠血清尿酸的同时,对实验动物血管内皮舒缩功能及主动脉形态方面有保护作用。

Objective To explore the effects and mechanism of Lishi Huoxue（ damp-dispersing and blood-activating） formula and two decomposed recipes Chinese herbal compound,on the vascular endothelial cells by detecting the serum levels of functional indicators of vascular endothelial cells and the aortic morphology of the rat model of hyperuricemia. Methods Male SD rats were randomly divided into seven groups： normal group,model group,benzbromarone group,Simiao Pill group,Lishi Huoxue formula（ LSHX） group,decomposed recipe I for clearing heat and damp（ recipe I） group,decomposed recipe Ⅱ for activating blood and resolving stasis（ recipe Ⅱ） group. The rat model of hyperuricemia was established by intragastrical（ i. g.） administration of yeaut and adenine. After successful modeling,rats of each medication group were administered i. g. with corresponding drugs,while normal group and model group were i. g. distilled water for consecutive 14 days. Then the serum contents of uric acid（ UA）and nitric oxide（ NO） were detected by ultraviolet-visible spectroscopy,and endothelin（ ET-1）,thromboxane B_2（ TXB_2） and 6-keto-PGF_（1α）were measured by radioimmunoassay. Besides,thoracoabdominal aorta of all rats were dissected and stained using HE and Masson. Results Compared with normal group,the serum level of UA,ET-1 and TXB_2 increased significantly in model group（ P 0. 05） and level of NO and 6-keto-PGF_（1α）decreased（ P 0. 05）. Compared with the model group,the serum contents of UA,ET-1 and TXB_2 showed significantly decreasing in LSHX group（ P 0. 05）,while in recipe I group UA and ET-1 decreased（ P 0. 05） and serum contents of NO,6-keto-PGF_（1α）in LSHX group,recipe ⅠandⅡ groups increased（ P 0. 05）. Furthermore,compared with LSHX group,the levels of TXB_2 in both recipe groups and ET-1 in recipe Ⅱ group showed on statistical difference. Although LSHX and the two partial prescriptions（ recipe I,II） protected against the injury of aorta,the whole prescription（ LSHX）was more effective than the two decomposed recipes. Conclusion LSHX formula could reduce the serum UA,and protect vascular endothelium and the aorta of rats with hyperuricemia.