糖肾清2号方对2型糖尿病模型小鼠肾功能的影响

Protective effect of Tangshenqing Formula Ⅱ on renal function of type 2 diabetic mice

目的观察糖肾清2号方对2型糖尿病模型小鼠肾功能的保护作用。方法采用自发性2型糖尿病模型KK-Ay小鼠,予高脂饲料诱导3周后,按血糖及体重水平随机分为模型组、缬沙坦组、糖肾清2号方大、中、小剂量组,以C57BL/6J小鼠设为正常组,每组各6只,连续干预治疗12周。分别在0、4、8、12周检测各组小鼠空腹血糖、尿微量白蛋白(Alb)、尿肌酐(Cr)、尿微量白蛋白/尿肌酐(ACR)及24 h尿蛋白,药物干预12周后实验结束,留取血清标本,全自动生化分析仪检测甘油三酯(TG)、总胆固醇(TC)、血清肌酐(Scr)、尿素氮(BUN)水平,酶联免疫吸附试验(ELISA)法检测血清β2微球蛋白(β2-MG)水平变化。结果与正常组比较,模型组小鼠24 h尿蛋白、ACR、Scr、TG、TC水平显著升高(P<0.05,P<0.01),BUN、β2-MG及肾重/体重变化不显著(P>0.05)。与模型组比较,缬沙坦组及糖肾清2号方大、中、小剂量组小鼠Scr、TG、ACR水平明显下降(P<0.05,P<0.01);24 h尿蛋白、TC、空腹血糖等指标有不同程度下降。结论糖肾清2号方可明显降低KK-Ay小鼠空腹血糖、Scr、TG水平,减轻糖尿病肾病(DN)肾损害,延缓DN发病进程。

Objective To observe the protective effect of Tangshenqing Formula II（ TSQF） on the renal function of mice with type 2 diabetes mellitus（ DM）. Methods KK-Ay mice,a spontaneous DM model,were fed with fat-enriched diet for three weeks. Based on their serum glucose（ Glu） and body weight（ BW）,the mice were divided into model group,valsartan group,high,middle,and low dose of TSQF（ high-,mid-,low- dose） group（ n = 6 each） and were treated for consecutive 12 weeks. And six C57 BL /6J mice were included into control group. At the timepoint of Week 0,4,8,12,Glu,urine albumin（ Alb）,urine creatine（ Cr）,Alb/Cr（ ACR） and 24-h urine protein（ pr /24 h） were detected. At the end of Week 12,all the mice were sacrificed and their serum samples were collected. And the serum levels of TG,TC,Scr,BUN and β2-MG were measured. Results Compared with the control group,the levels of pr /24 h,ACR,Scr,TG and TC in the model group significantly increased（ P 0. 05 or P 0. 01）,While BUN,β2-MG and ratio of kidney weight to body weight showed no statistcal differences（ P 0. 05）. Compared to the model group,the levels of Scr,TG and ACR in valsartan group and three TSQF groups significantly decreased（ P 0. 05 or P 0. 01）,and those of TC,Glu,and pr /24 h decreased at different degree. Conclusion TSQF could reduce the serum levels of Glu,Scr and TG of kk-Ay mice,and alleviate the kidney injuries to reduce the progression of diabetic nephropathy.