GLP体系下比格犬临床病理学指标正常参考值的建立

Establishment of normal reference ranges for clinical pathology indexes of Beagle dogs in GLP system

目的建立实验室操作规范(GLP)体系下比格(Beagle)犬临床病理学指标的正常参考值范围。方法检测117只比格犬的16项血清生化指标、17项血液学指标及3项凝血指标,计算并比较各项平均值及正常参考值范围。结果血清生化指标仅有碱性磷酸酶(alkaline phosphatase,ALP)一项雌雄性别差异显著(P<0.01),且雄性高于雌性;血液学指标中红细胞(red blood cell,RBC)、血红蛋白(hemoglobin,HGB)、红细胞压积(hematocrit,HCT)3项雌雄性别差异显著(P<0.01),均为雄性低于雌性;凝血指标无雌雄性别差异。结论比格犬仅有个别临床病理指标存在雌雄性别差异,因此在毒性实验中,可考虑将雌雄动物检测结果合并进行统计分析。该研究建立了GLP体系下比格犬的临床病理学指标正常参考值,为毒理学研究提供了参考数据。

Objective To establish the normal reference ranges of clinical pathology for Beagle dogs in the Good Laboratory Practice （GLP） system. Method Sixteen biochemical indexes, seventeen hematological indexs and three coagulation function indexes of 117 Beagle dogs were detected, and the mean value of each index and the normal reference ranges were calculated and compared. Results Only alkaline phosphatase （ALP） from the biochemical items was significantly different between males and females （P 〈 0.01 ）, which was higher among males than among females. Three in- dexes of hematology were significantly different between males and females（ P 〈 0.01 ） ,with red blood cell （RBC）, hemo- globin （HGB） and hematocrit（HCT） lower among males than among females. The coagulation function items were not signif- icantly different between the two sexes. Conclusion Some indexes of clinical pathology were significantly different between males and females, which should be considered during statistic analysis on toxicity. Our study has established the normal reference range of clinical pathology for Beagle dogs in the GLP system, which provides reference for toxicity tests.